Isolation and characterization of Klebsiella pneumoniae unencapsulated mutants. |
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| Authors: | V J Benedí , B Ciurana, J M Tom s |
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| Affiliation: | Departamento de Biología y Ciencias de la Salud (Laboratorio de Microbiologia), Universidad de las Islas Baleares, Palma de Mallorca, Spain. |
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| Abstract: | Klebsiella pneumoniae mutants were obtained after UV irradiation and negative selection with anticapsular serum. Unencapsulation, rather than expression of a structurally altered capsule, was found in the mutants. The mutant strains showed no alterations in their outer membrane proteins and lipopolysaccharide, and a great similarity with the wild type in the properties tested (serum resistance, antimicrobial sensitivity, and lipopolysaccharide-specific bacteriophage sensitivity), with the exception of a higher cell surface hydrophobicity and resistance to bacteriophage FC3-9. |
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