Nucleostemin 特异性RNA干扰对肿瘤细胞增殖能力的影响

目的：检测肿瘤细胞Nucleostemin(NS)的表达，研究NS特异性RNA干扰对Hela细胞体内外增殖的影响。方法：提取6种肿瘤细胞总RNA，用RT-PCR和Northern blot方法检测NS的表达。用NS特异性siRNA表达载体转染Hela细胞，观察转染的Hela细胞(简称NS-SiRNA-Hela细胞)体内外增殖的变化。结果：6种肿瘤细胞中NS显高表达。体外培养的NS-siRNA-Hela细胞中NS表达显降低，Gn／G，期细胞百分率显升高。体内致瘤实验显示，NS-siRNA-Hela细胞在裸鼠体内增殖显降低。结论：NS在肿瘤细胞中高表达具有普遍性。NS特异性RNA干扰使Hela细胞进入S期受阻，并可明显降低Hela细胞体内外的增殖能力。

NUCLEOSTEMIN SPECIFIC RNA IN SEVERAL TUMOR CELLS INFLUENCED THE CELLS PROLIFERATION

Objective: To examine nucleostemin (NS) expression in tumor cells, and to study the effect of NS specific RNA interference to the cell proliferation in Hela cells. Methods: Total RNA was extracted from 6 cultured tumor lines, the NS expression level was measured by RT-PCR and Northern blot. A NS-specific siRNA expression vector was constructed to transfect Hela cell (NS-sirNA-Hela),and the proliferation of the cell was observed. Results: NS was highly expressed in 6tumor cells. NS expression level in the NS-siRNA-Hela cells was remarkably reduced, and the percentage of G 0/G 1 cells increased. The neoplasm forming ability in nude mice by the NS-siRNA-Hela cell decreased. Conclusion: NS is highly expressed among tumor cells. NS-specific siRNA inhibit the entry of the cell cycle into the S phase, and remarkably reduce the proliferation ability of Hela cells in vitro and in vivo.