| 咳尔康口服液中多糖脱蛋白方法研究 |
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| 引用本文： | 巩丽虹,李孟全,徐红纳,石秀梅,常希文,金秀东. 咳尔康口服液中多糖脱蛋白方法研究[J]. 安徽医药, 2010, 14(6): 635-636. DOI: 10.3969/j.issn.1009-6469.2010.06.006 |
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| 作者姓名： | 巩丽虹 李孟全 徐红纳 石秀梅 常希文 金秀东 |
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| 作者单位： | 牡丹江医学院药学系,牡丹江,157011;哈尔滨市医药工程学校药科教研室,哈尔滨,150000 |
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| 基金项目： | 黑龙江省教育厅攻关项目 |
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| 摘 要： | 目的研究咳尔康口服液中多糖脱蛋白的方法。方法以糖保留率和蛋白去除率为指标,对氯化钙法、盐酸法、三氯乙酸法、Sevag法4种脱蛋白方法进行比较。结果通过正交试验确定了Sevag法的最佳条件为：样品：（氯仿-正丁醇）=3：1,氯仿：正丁醇=3：1,振荡30min,添加试剂2次。结论 Sevag法脱蛋白效果最好。
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| 关 键 词： | 咳尔康口服液 多糖 脱蛋白 Sevag法 |
Methods of deproteinization from polysaccharide in keerkang oral liquid |
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| GONG Li-hong,LI Meng-quan,XU Hong-na,SHI Xiu-mei,CHANG Xi-wen,JIN Xiu-dong. Methods of deproteinization from polysaccharide in keerkang oral liquid[J]. Anhui Medical and Pharmaceutical Journal, 2010, 14(6): 635-636. DOI: 10.3969/j.issn.1009-6469.2010.06.006 |
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| Authors: | GONG Li-hong LI Meng-quan XU Hong-na SHI Xiu-mei CHANG Xi-wen JIN Xiu-dong |
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| Affiliation: | (Department of Pharmacy,Mudanjiang Medical University,Mudanjiang 15000,China) |
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| Abstract: | Aim To study the methods of removing protein from polysaccharide of keerkang oral liquid.Methods With the ratios of polysaccharides retention and protein rejection as the indexes,calcium chloride method,hydrochloric acid method,the trichloroacetic acid method and Sevag method were compared.Results The optimum conditions of Sevag method determined by orthogonal test were as follows：3：1 ratio of sample：chloroform：n-butanol,3：1 ratio of chloroform-n-butanol,30 min stirring time,2 times adding agent.Conclusion Sevag method had the best effect on deproteinization. |
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| Keywords: | keerkang oral liquid polysaccharide deproteinization sevag method |
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