IL-13 基因+2044G/A 多态性与儿童哮喘风险和血清IL-13 水平的相关性研究

目的探讨IL-13基因+2044G/A多态性与儿童哮喘发病风险及哮喘儿童血清IL-13水平的关系。方法采用PCR-RFLP和ELISA方法,对90例支气管哮喘患儿(哮喘组)和82例健康儿童(对照组)IL-13基因+2044G/A多态性和血清IL-13水平进行检测,计算基因型、等位基因频率及其所对应的血清IL-13水平。结果哮喘组和对照组IL-13+2044G/A多态性3种基因型的分布差异有统计学意义(P0.001);与GG基因型比较,GA基因型儿童哮喘风险增加3.52倍(95%CI:1.82~6.82),AA基因哮喘风险增加3.71倍(95%CI:1.06~12.97)。哮喘组A等位基因频率为36.1%,高于对照组的18.3%,差异有统计学意义(P0.001)。与G等位基因比较,A等位基因携带儿童哮喘风险增加2.53倍(95%CI:1.53~4.16)。哮喘组患儿血清IL-13水平为151.82(134.33~166.68)ng/L,对照组为79.00(58.74~111.09)ng/L,两组差异有统计学意义(P0.001)。哮喘组GG、GA和AA基因型的血清IL-13水平均分别高于对照组,差异有统计学意义(P均0.01)。哮喘组GG、GA和AA 3种基因型之间血清IL-13水平的差异也有统计学意义(P0.01),其中GA和AA基因型血清IL-13水平高于GG基因型。结论 IL-13基因+2044G/A多态性与支气管哮喘儿童血清IL-13水平具有关联性,且A等位基因可能造成了哮喘儿童血清高IL-13水平,携带A等位基因的儿童哮喘风险增加。

Association ofIL-13 gene + 2044 G/A polymorphism to the risk of pediatric asthma and its relevancy to serum levels of IL-13

Objective To investigate the relationship between IL-13 gene + 2044G / A polymorphism to the risk of asthma in children and its relevancy of serum IL-13 levels.Methods Ninty cases of children with bronchial asthma (asthma group) and 82 healthy children (control group) were recruited. PCR-RFLP and ELISA were used to detectIL-13 gene 2044G?>?A polymorphism and serum IL-13 levels. Genotype, allele frequencies and the corresponding serum IL-13 level were calculated.χ2 test andt-test were used to compare the genotype, allele frequency, and serum IL-13 level between the two groups.Results The distribution ofIL-13 gene 2044G?>?A polymorphism showed signiifcant difference between the two groups. Compared with AA genotype and A allele, children with GG genotype and G allele were associated with an increased risk of bronchial asthma, and theOR value were 3.706 (95%CI:1.059~12.976) (P<0.001) and 2.525 (95%CI:1.532~4.159) (P<0.001). The level of serum IL-13 is higher in asthma group than in control group (Z= 7.899,P<0.001), and the serum IL-13 level of GA and AA in asthma group is higher than that in children with GG genotype (Z= 5.555,P<0.001;Z= 3.541,P<0.001;Z= 2.777, P<0.05).Conclusion There is association betweenIL-13 2044G?>?A polymorphism and serum IL-13 level in children' with bronchial asthma. And A allele maybe associated with higher serum IL-13 level in children with asthma.