白介素-18在急性胰腺炎大鼠中性粒细胞凋亡中的作用

目的:探讨白介素-18(IL-18)在重症急性胰腺炎(SAP)时外周血中性粒细胞(PMN)凋亡中的作用.方法:SD大鼠60只,随机分为2组,每组30只,分别建立大鼠急性坏死性胰腺炎组(ANP)及假手术组(SO)模型,在制模后3、6、12h分期分批处死大鼠,抽取下腔静脉血测定血淀粉酶含量及血清中IL-18的含量,作中性粒细胞分离并检测外周血中性粒细胞凋亡率;同时对大鼠胰腺组织行病理切片HE染色,并对胰腺组织进行病理评分.结果:ANP组中性粒细胞(PMN)凋亡率较SO组明显降低,并随着时间的延长逐渐明显(P<0.01),至术后12h降至2.15%±0.45%;ANP组血清中IL-18的含量较SO组明显升高,而且随着时间的延长更明显(P<0.01),在12h达到4705.70±296.30;ANP组PMN的凋亡率与血清中IL-18的水平呈负相关(r=-0.78,P<0.01).结论:ANP组外周血PMN的凋亡明显延迟,血清中IL-18的含量明显增高,IL-18可能在急性胰腺炎外周血的PMN凋亡中发挥作用.

Role of IL-18 in peripheral neutrophil apoptosis in rats with sever acute pancreatitis

AIM:To examine the role of interleukin-18 (IL18) in apoptosis of peripheral blood neutrophils (PMNs) in rats with severe acute pancreatitis (SAP).METHODS:Sixty SD rats were randomly divided into two groups:SAP group and sham operation (SO) group.At 3,6 and 12 h after SAP induction,blood samples were collected from the inferior vena cava to measure serum amylase and IL 18;PMNs were separated to detect their apoptosis;and pancreatic specimens were taken for pathological examination.RESULTS:PMN apoptosis rate decreased remarkably in the SAP group than in the SO group,and the decrease became more obvious over time (P<0.01).Serum levels of IL-18 in the SAP group increased significantly compared with those in the SO group (P<0.01).There was a negative correlation between the apoptosis rate of PMNs and serum levels of IL-18 in SAP rats (r=-0.742,P<0.01).CONCLUSION:Compared to control rats,PMN apoptosis was obviously delayed and serum levels of IL-18 remarkably increased in SAP rats,indicating that IL-18 may play an important role in peripheral neutrophil apoptosis in SAP.