| RP—HPLC测定海南产胡椒根中胡椒碱的含量 |
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| 引用本文： | 施蕊,华燕. RP—HPLC测定海南产胡椒根中胡椒碱的含量[J]. 中国民族民间医药杂志, 2010, 19(1): 39-40 |
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| 作者姓名： | 施蕊 华燕 |
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| 作者单位： | 西南林学院西南山地森林资源保育与利用省部共建教育部重点实验室,云南昆明650224 |
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| 摘 要： | 目的：采用RP—HPLC首次建立了海南产胡椒根中胡椒碱的含量测定方法。方法：分析柱采用Eclipse XDB-C18（4．6＊ 150mm，5um）色谱柱，流动相为甲醇-水（V：V=75：25）；流速：1．0mL／min；检测波长：343nm。结果：胡椒碱的线性范围分别为0．1～1．0mg／ml；相关系数为0．9997。平均加样回收率分别为9．4％，98．7％，100．9％；RSD为2．1％，1．6％，2．3％（n=9）。结论：采用RP—HPLC同时测定海南产胡椒根中胡椒碱的含量，该法简单、可靠，可作为控制胡椒根药材质量的参考标准。
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| 关 键 词： | 胡椒根 胡椒碱 RP—HPLC |
RP-HPLC Determination of Piperine in Roots of Piper nigrum Linn. from Hainan |
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| SHI Rui,HUA Yan Key Laboratory for Forest Resources Conservation , Use in thesouthwest Mountains of China,Southwest Forestry University Yunnan Kunming. RP-HPLC Determination of Piperine in Roots of Piper nigrum Linn. from Hainan[J]. Chinese Journal of Ethnomedicine and Ethnopharmacy, 2010, 19(1): 39-40 |
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| Authors: | SHI Rui HUA Yan Key Laboratory for Forest Resources Conservation Use in thesouthwest Mountains of China Southwest Forestry University Yunnan Kunming |
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| Affiliation: | Ministry of Education |
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| Abstract: | Objective:To establish a method for determination of piperine in Roots of Piper nigrum Linn.from Hainan by RP-HPLC.Method: Thesample wasseparated on Eclipse XDB-C18(4.6*150mm,5um) column,the mobile phase was methanol-water(V:V=75:25) at a flow rate of 1.0ml/min,and the detection wavelength was 343 nm.Results: The linear range of piperine was 0.1-1.0 mg/ml with the correlation coefficients 0.9997.The average recoveries of piperine were 99.4%,98.7%,100.9%.RSD were 2.1%,1.6%,2.3%(n=9).Conclusion: The method is... |
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| Keywords: | Roots of Piper nigrum Linn. piperine RP-HPLC |
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