Schwann cell-conditioned medium supports neurite outgrowth and survival of spinal cord neurons in culture |
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Authors: | E P Bosch J G Assouline N J Pantazis R Lim |
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Institution: | Department of Neurology, University of Iowa College of Medicine, Iowa. |
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Abstract: | The effect of Schwann cell-conditioned medium (SCM) on the development in vitro of spinal cord neurons was studied. Spinal cord neurons from 18-day-old rat embryos were cultured in serum-free conditioned medium obtained from confluent rat Schwann cells. In cultures fed SCM, the cells developed typical neuronal morphology and were identified by indirect immunofluorescence using a monoclonal antibody to neurofilament protein. SCM stimulated neurite outgrowth and supported survival of spinal cord neurons. Preliminary characterization suggests that the neurotrophic factor in SCM appears to be a protein with a molecular weight greater than 8000 daltons. |
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