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| 山酮类化合物抑制人脑胶质瘤细胞增殖活性及作用机制探讨 | |
| 引用本文： | 韩懿岚,王思明,李晓峰,董玫,路新华,史清文,丛斌,谷建平. 山酮类化合物抑制人脑胶质瘤细胞增殖活性及作用机制探讨[J]. 中国药理学通报, 2011, 27(9): 1227-1230. DOI: 10.3969/j.issn.1001-1978.2011.09.011 |
| 作者姓名： | 韩懿岚 王思明 李晓峰 董玫 路新华 史清文 丛斌 谷建平 |
| 作者单位： | 1. 河北医科大学法医学系 2. 河北医科大学药学院,天然药物化学教研室,河北,石家庄,050017 3. 河北医科大学药学院,天然药物化学教研室,河北,石家庄,050017;华北制药集团新药研究开发有限责任公司微生物药物国家工程研究中心,河北,石家庄,050015 |
| 基金项目： | 国家自然科学基金资助项目，河北省自然科学基金资助项目，河北省科技支撑资助项目 |
| 摘 要： | 目的检测3种山酮类化合物对人脑肿瘤细胞(T-98、U251SP和KT)增殖的抑制作用,并进一步探讨其可能的作用机制。方法研究从3种微生物次生代谢产物中纯化的山酮类化合物对人脑肿瘤细胞增殖的抑制作用。MTT比色法检测细胞增殖抑制率;利用双荧光素酶报告基因检测系统检测p53和Bax报告基因的表达;采用Western blot分析与凋亡相关的蛋白表达变化。结果 Austocystin H对KT细胞的增殖显示极强的抑制活性,呈剂量依赖性,IC50仅为0.69μmol.L-1;Austocystin H可诱导KT细胞内Bax报告基因表达(P<0.01);Western blot分析结果显示由Austocystin H处理的KT细胞中的p53和Bax的表达与对照组相比增加(P<0.05)。结论 Austocystin H具有极强的抑制人脑肿瘤细胞增殖作用,其作用机制可能与其诱导肿瘤细胞凋亡有关。 |
| 关 键 词： | 山酮 Austocystin H 脑肿瘤细胞 增殖 报告基因 细胞凋亡 |
Study on anti-growth activity and its mechanism in glioma cells by xanthones |
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| HAN Yi-lan,WANG Si-ming,LI Xiao-feng,DONG Mei,LU Xin-hua,SHI Qing-wen,CONG Bin,GU Jian-ping. Study on anti-growth activity and its mechanism in glioma cells by xanthones[J]. Chinese Pharmacological Bulletin, 2011, 27(9): 1227-1230. DOI: 10.3969/j.issn.1001-1978.2011.09.011 | |
| Authors: | HAN Yi-lan WANG Si-ming LI Xiao-feng DONG Mei LU Xin-hua SHI Qing-wen CONG Bin GU Jian-ping |
| Affiliation: | HAN Yi-lan1,WANG Si-ming2,LI Xiao-feng1,DONG Mei1,LU Xin-hua2,3,SHI Qing-wen2,CONG Bin1,GU Jian-ping1(1.Dept of Forensic Medicine,Hebei Medical University,Hebei Key Laboratory of Forensic Medicine,Shijiazhuang 050017,China,2.Dept of Medicinal Natural Product Chemistry,School of Pharmaceutical Sciences,3.New Drug Research and Development Center,North China Pharmaceutical Group Corporation,National Microbial Medicine Engineering and Research Center,Shijiazh... |
| Abstract: | Aim To discover the anticancer effect of three xanthones and to study its mechanisms.Methods Anticancer xanthone compounds from microbial metaboites were screened by measuring the antiproliferation effects on human glioma cell lines.The cell viabilities were examined by MTT assay.KT cells were transiently cotransfected by pG53-Luc,pGBax-Luc reporter plasmid and SV-40-Rluc plasmid.The effects of Austocystin H of luciferase activity expression were detected by Dual-Luciferase Reporter Assays.The protein expre... |
| Keywords: | Austocystin H |
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