维生素C和维生素E对汞毒性的拮抗作用

目的 探讨维生素C(VC)和维生素E(VE)对汞毒性的影响。方法 48只Wistar大鼠随机分成6组：对照组、低、中、高剂量染汞组、VC 高剂量染汞组、VE 高剂量染汞组。注射后48h处死大鼠，采集肝脏和肾皮质样品。测定肝脏和肾皮质的汞、丙二醛(MDA)和谷胱甘肽(GSH)含量及谷胱甘肽过氧化物酶(GSH-Px)活性。结果 肝和肾皮质汞含量随染汞剂量增加而升高，且高剂量染汞组显著高于对照组。所有组肾皮质汞含量显著高于肝汞。中剂量染汞组肝GSH含量、GSH-Px活性显著高于对照组。低、中剂量染汞组肾皮质GSH含量、GSH-Px活性较对照组显著升高，中、高剂量染汞组肾皮质MDA含量显著高于对照组。VC和VE预处理组肾皮质MDA含量显著低于高剂量染汞组，肾皮质GSH含量、GSH-Px活性和肝脏中GSH含量显著高于对照组、高剂量染汞组。结论 VC和VE对汞毒性具有一定的拮抗作用。

Effects of VC and VE on toxicity of mercury

Objective To study the effects of vitamin C(VC) and vitamin E(VE) pretreatment on the toxicity of mercury (Hg).Methods Forty-eight Wistar rats were divided into six groups randomly, control group,three Hg groups,VC or VE pretreatment groups.At 48 hours after the last injection,the animals were sacrificed,and the liver and renal cortex were collected.Hg,malondialdehyde (MDA),glutathione (GSH) contents and glutathione-peroxidase (GSH-Px) activities in the liver and renal cortex were measured.Results Hg contents in the liver and renal cortex increased with Hg dose increasing,and high-dose Hg group was significantly higher than the control group.Hg contents in the renal cortex of all groups were higher than those in the liver.GSH contents and GSH-Px activities in the liver of middle-dose Hg group were higher significantly than the control values.GSH contents and GSH-Px activities in the renal cortex of low and middle dose Hg groups were higher significantly than the control values.MDA contents in the renal cortex of middle and high dose Hg groups were higher significantly than the control values.MDA contents in the renal cortex of VC and VE pretreatment groups were lower significantly than those of the high-dose Hg group.GSH contents,GSH-Px activities in the renal cortex and GSH contents in the liver of VC and VE pretreatment groups increased significantly as compared with those of the control and high-dose Hg groups.Conclusion VC and VE could protest against the toxicity induced by Hg.