26例有偿献血员HIV-1基因分型与变异特征

目的分析某地区有偿献血人员中流行的人免疫缺陷病毒1型（HIV-1）gag、pol、env基因亚型及基因变异特征。方法提取HIV-1感染者外周血单核细胞（PBMC）DNA,经巢式PCR（NestedPCR）扩增gag（p17-p24）、pol（PR＊RT）、env（C2-V5）基因片段,纯化测序后用MEGA5．0等生物学软件对核苷酸序列进行分析。结果23份样本为B亚型,2份为B亚型与C亚型重组,1份为CRF01-AE与B亚型重组。PR区未发现蛋白酶抑制剂主要耐药性突变,RT区检测到核苷类逆转录酶抑制剂耐药性突变M184V和非核苷类逆转录酶抑制剂耐药性突变K101E,G190A。结论流行于该地区的HIV-1毒株以B亚型为主。大多数毒株对常规抗病毒药物仍然敏感,使用HARRT治疗方案依然有效。CXCR4型辅助受体的毒株顶端四肽多为GPGR（91．7％）,提示GPGR可能与疾病的进展有关。

Genotyping and variability of HIV-1 in 26 cases of paid blood donors

Objective To analyze the genome mutations of HIV-1 gag, pol and env genes from HIV-infected paid blood donors in rural central China. Methods DNA was extracted from peripheral blood mononuclear ceils, gag（p17-p24）,pol（PR-RT）,env（C2-V5） genes were amplified by nested polymerase chain reaction （PCR） , purified products were sequenced, and sequence data was analyzed by MEGA5.0 soft wares. Results Twenty-three samples were subtype B, two samples were recombinant of subtype B and subtype C, one sample was recombinant of subtype CRF01_AE and subtype B. PI major resistance mutations were not found in the PR region. M184V, KIOIE and G190A were detected in the RT region, respectively. Conclusion Subtype B was the major HIV circulating genetic forms in this area. Most strains were sensitive to high active anti-retroviral therapy （ HARRT）. 91.7% V3 loop tip motifs of X4-tropic strains was GPGR. It showed that GPGR might be associated with accelerate disease progression to AIDS.