| 山楂叶总黄酮对非酒精性脂肪性肝炎大鼠肝组织CYP2E1表达的影响 |
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| 引用本文： | 陈芝芸,刘红,严茂祥,何蓓晖. 山楂叶总黄酮对非酒精性脂肪性肝炎大鼠肝组织CYP2E1表达的影响[J]. 中华中医药杂志, 2010, 0(1) |
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| 作者姓名： | 陈芝芸 刘红 严茂祥 何蓓晖 |
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| 作者单位： | 浙江省中医院消化病研究室; |
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| 基金项目： | 浙江省自然基金资助项目(No.Y2004310)~~ |
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| 摘 要： | 目的:观察细胞色素P4502E1(CYP2E1)在高脂饮食诱导的非酒精性脂肪性肝炎(NASH)大鼠肝组织中的表达及山楂叶总黄酮(TFHL)对其的影响。方法:以高脂饮食喂养12周建立NASH大鼠模型,分别以250、125mg/kg体质量/d的TFHL和195.4mg/kg体质量/d的易善复进行干预,观察大鼠肝组织的病理改变,肝组织匀浆MDA含量和SOD、GSH-Px的活性,RT-PCR和免疫组化法检测CYP2E1基因mRNA和蛋白在肝组织中表达。结果:①NASH模型组大鼠肝组织出现重度脂肪变、不同程度的炎细胞浸润及坏死灶,肝组织MDA含量较正常组大鼠显著增高,肝匀浆SOD、GSH-Px的活性明显降低,肝组织CYP2E1基因mRNA和蛋白表达显著增强。②肝组织CYP2E1基因的表达与MDA含量呈明显正相关,与SOD和GSH-Px活性则呈明显负相关。③TFHL高、低剂量组大鼠肝组织炎症活动程度、肝匀浆MDA含量、肝组织CYP2E1基因mRNA和蛋白表达均较模型组大鼠显著降低,而肝匀浆SOD、GSH-Px的活性则较模型组大鼠明显增高。结论:CYP2E1参与高脂饮食诱导的大鼠NASH的发生发展,TFHL能抑制CYP2E...
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| 关 键 词： | 山楂叶总黄酮 非酒精性脂肪性肝炎 细胞色素CYP2E1 |
Effect of TFHL on expression of CYP2E1 in liver tissue of rats with NASH |
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| CHEN Zhi-yun,LIU Hong,YAN Mao-xiang,HE Bei-hui. Effect of TFHL on expression of CYP2E1 in liver tissue of rats with NASH[J]. China Journal of Traditional Chinese Medicine and Pharmacy, 2010, 0(1) |
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| Authors: | CHEN Zhi-yun LIU Hong YAN Mao-xiang HE Bei-hui |
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| Affiliation: | Department of Digestion Diseases;Zhejiang College of Traditional Chinese Medicine;Hangzhou 310006;China |
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| Abstract: | Objective:To observe the expression of cytochrome P4502E1 (CYP2E1) in the liver tissue of rats with non-alcoholic steatosis hepatitis (NASH) induced by high-fat diet, and effect of total flavonoids of Chinese hawthorn leaf(TFHL). Methods: The model rats of NASH were induced by 12 weeks' high-fat diet, and treated with TFHL and Essentiale. The pathological changes of rats liver were observed, the amounts of liver MDA, and the activities of SOD, GSH-Px were detected, the mRNA and protein expressions of CYP2E1... |
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| Keywords: | Total flavones of hawthorn leafon NASH CYP2E1 |
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