小鼠脑微血管内皮细胞的体外培养

目的 探讨脑微血管内皮细胞的体外培养方法并进行细胞超微结构研究及组织型纤溶酶原激活物（ＴＰＡ）活性测定。方法 取新生小鼠脑组织，通过匀浆、过筛、胶原酶消化、差速粘附等技术对鼠脑微血管内皮细胞进行原代培养，待细胞铺满瓶底时，用０．１２５％胰酶－０．０２％ＥＤＴＡ消化，离心收集内皮细胞，进行传代培养。原代、传代各取８例，吸取培养液用酶联免疫吸附试验测试ＴＰＡ活性。结果 经Ⅷ因子相关抗原免疫组织化学鉴定

Cultivation of Mouse Brain Microvascular Endothelial Cells in Vitro

Objective To study the method of cultivation and to observe the ultrastructure characteristics of mouse brain microvascular endothelial cells. Furthermore,the activity of tissue|type plasminogen activator(TPA) was detected. Methods Primary culture brain microvascular endothelial cells isolated from mouse brain were prepared by homogenate,filtration,collagenase digestion,differential conglutination. Cells from a confluent cell monolayer digested by 0.125% trypsin|0.02% EDTA were used for passage. The activity of TPA was detected by using enzyme|linked immunosorbent assays(ELISA). Results It was verified that cultivated cells synthesized and excreted TPA were vascular endothelial cells by morphology,ultrastructures and immunohistochemistry studies. Conclusion This results would provide a useful tool for studying diseases related to cerebral vessels in vitro.;