| 壳聚糖促进体外培养SD乳鼠视网膜神经细胞生长的初步研究 |
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| 引用本文： | 杨红,赵燕. 壳聚糖促进体外培养SD乳鼠视网膜神经细胞生长的初步研究[J]. 华中科技大学学报(医学版), 2010, 39(1). DOI: 10.3870/j.issn.1672-0741.2010.01.029 |
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| 作者姓名： | 杨红 赵燕 |
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| 作者单位： | 1. 华中科技大学同济医学院附属同济医院眼科,武汉,430030
2. 武汉市爱尔眼科医院 |
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| 基金项目： | 湖北省科委重点科技研究项目 |
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| 摘 要： | 目的 研究壳聚糖对体外培养视网膜神经细胞生长的影响.方法 建立体外培养的SD乳鼠视网膜神经细胞.用MTT法检测0.065%、1.25%、2.5%、5%、10%、15%不同浓度壳聚糖对视网膜神经细胞生长的影响并设立空白对照组.结果 1.25%、2.5%、5%、10%壳聚糖对视网膜神经细胞生长有明显的促进作用,且10%浓度壳聚糖最适宜神经细胞生长.结论 壳聚糖有较好的促进视网膜神经细胞生长的作用,有必要对其进行更深入的研究.
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| 关 键 词： | 视网膜神经细胞 壳聚糖 体外培养 |
Chitosan Promoting the Growth of in vitro Cultured Retinal Nerve Cells of SD Baby Rats |
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| Yang Hong,Zhao Yan. Chitosan Promoting the Growth of in vitro Cultured Retinal Nerve Cells of SD Baby Rats[J]. Journal of Huazhong University of Science and Technology(Health Sciences), 2010, 39(1). DOI: 10.3870/j.issn.1672-0741.2010.01.029 |
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| Authors: | Yang Hong Zhao Yan |
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| Affiliation: | Yang Hong,Zhao Yan Department of Ophthalmology,Tongji Hospital,Tongji Medical College,Huazhong University of Science , Technology,Wuhan 430030,China |
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| Abstract: | Objective To study the effect of chitosan on the growth of the in vitro cultured retinal nerve cells.Methods The retinal nerve cells of SD baby rats were cultured in vitro.The effects of chitosan at 0.065%,1.25%,2.5%,5%,10%,15% on the growth of the retinal nerve cells were measured by MTT method.The blank control group was created.Results 1.25%,2.5%,5%,and 10% chitosan could obviously promote the growth of retinal nerve cells,and 10% chitosan was the optimal concentration for the growth of nerve cells.Concl... |
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| Keywords: | retinal nerve cell chitosan in vitro culture |
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