六白菖砂粒剂对颈椎病动物模型骨形态发生蛋白2及LIM矿化蛋白1的影响

背景:骨形态发生蛋白2与LIM矿化蛋白1在成骨细胞分化、成熟中有重要作用,与颈椎病的发生有很大联系。目的:观察六白菖砂粒剂对家兔颈椎病模型外周血清中骨形态发生蛋白2、LIM矿化蛋白1的影响。方法:将50只成年健康雄性大耳白兔随机分为5组。除正常对照组外采用蹲坐低头位制作颈椎病动物模型。颈复康对照组每天以颈复康0.25g灌胃,六白菖砂粒剂高剂量组和低剂量组每天分别以0.5g、0.25g灌胃,正常对照组和模型对照组予同等容量的蒸馏水灌胃。与4周、12周测量动物颈椎骨密度,12周时取椎体检测离体骨密度,并检测外周血清中骨形态发生蛋白2、LIM矿化蛋白1因子水平。结果与结论:六白菖砂粒剂高剂量组中骨形态发生蛋白2、LIM矿化蛋白1水平显著高于空白对照组、颈椎病模型对照组和六白菖砂粒剂低剂量组(P<0.05);颈椎病模型对照组中骨形态发生蛋白2、LIM矿化蛋白1水平明显低于正常对照组、颈复康颗粒对照组和六白菖砂粒剂高剂量组(P<0.05),而与六白菖砂粒剂低剂量组相比差异无显著性意义(P>0.05)。提示六白菖砂粒剂是通过提高家兔血清骨形态发生蛋白2、LIM矿化蛋白1水平对颈椎病产生疗效。

Liubaichangsha granule effects on bone morphogenetic protein 2 and LIM mineralization protein 1 in cervical disease rabbits

BACKGROUND：Bone morphogenetic protein 2 and LIM mineralization protein 1 play an important role in osteoblast differentiation and maturation,which are greatly associated with cervical diseases.OBJECTIVE：To observe the influence of Liubaichangsha granule on bone morphogenetic protein 2 and LIM mineralization protein 1 in the peripheral serum of cervical disease rabbit models.METHODS：Fifty adult rabbits were randomly divided into five groups.Cervical disease models were made through keeping squatting and head-bending position in all the groups except for the normal control group.Intragastric administration of 0.25 g Jingfukang granule,0.5 g Liubaichangsha granule and 0.25 Liubaichangsha granule per day was performed respectively in the Jingfukang group,low-dose and high-dose Liubaichangsha groups.The same volume of diluted water was given in the normal control and model groups.All the rabbits were examined with roentgenogram and dual energy radiograph absorptiometry for the cervical vertebrae in the 4th and 12th weeks,the pathology of the cervical intervertebra discs were measured in the 12th week,and bone morphogenetic protein 2 and LIM mineralization protein 1 levels in the peripheral blood serum were determined and observed in the 12th week.RESULTS AND CONCLUSION：The bone morphogenetic protein 2 and LIM mineralization protein 1 levels in the high-dose Liubaichangsha group were significantly higher than those in the normal control,model and low-dose Liubaichangsha groups（P〈0.05）.The model group had a lower levels of bone morphogenetic protein 2 and LIM mineralization protein 1 as compared with the normal control,Jingfukang and high-dose Liubaichangsha groups（P〈0.05）,but not differ from the low-dose Liubaichangsha group（P〈0.05）.These findings indicate that Liubaichangsha granule can increase the levels of bone morphogenetic protein 2 and LIM mineralization protein 1 so as to cure cervicaldiseases.