几种药物干预骨质疏松模型大鼠股骨的剪切力学特性☆

背景:剪切力学性能指标是对药物治疗后骨质疏松质量评价的一种可靠方法。目的:建立骨质疏松动物模型,比较不同药物干预骨质疏松模型大鼠后的剪切力学性能变化。方法:将60只大鼠分别设为正常对照组10只,其余50只去除双侧卵巢法复制骨质疏松动物模型,再随机分为5组:模型组不用药,复方中药组大鼠每日给服中药0.9g/kg、阿仑膦酸钠组大鼠每日给服阿仑膦酸钠1mg/kg、维生素K组大鼠每日给服维生素K0.1mg/kg、钙剂组大鼠每日给服逸得乐2mg/kg。各组大鼠股骨在电子万能试验机上进行剪切力实验。结果与结论:正常对照组、复方中药组、阿仑膦酸钠组、维生素K组大鼠股骨最大剪切力、最大剪应力均显著高于模型组(P<0.05)。钙剂组大鼠最大剪切力、最大剪应力与模型组比较差异无显著性意义。复方中药组大鼠最大剪切力、最大剪应力与正常对照组比较,差异无显著性意义。提示:中药、西药、维生素K治疗动物骨质疏松模型大鼠均有一定疗效,钙剂效果不明显,中药的效果最好。

Several drugs intervene rat models of osteoporosis：Cutting mechanical characteristics of the thighbone

BACKGROUND：Cutting mechanical performance indexes are a reliable method for quantitative evaluation of osteoporosis after drug treatment.OBJECTIVE：To establish animal models of osteoporosis and to compare the changes in cutting mechanical performance in rat models of osteoporosis after different drug interventions.METHODS：Fifty rats were selected to prepare models of osteoporosis by removal of both ovaries,and then randomized into five groups：model group with no treatment,compound Chinese medicine group with 0.9 g/kg Chinese medicine per day,alendronate group with 1 mg/kg alendronate per day,vitamin K group with 0.1 mg/kg vitamin K per day,calcium supplement group with 2 mg/kg calcium supplement per day.Another 10 rats served as normal control group.Electronic universal testing machine was used for shear force determination.RESULTS AND CONCLUSION：Maximum shear force and maximum shear stress in the model group were lower than those in the normal control,compound,alendronate and vitamin K groups（P〈0.05）.No difference was found in the maximum shear force and maximum shear stress between the model and calcium supplement groups and between the compound and normal control groups.These results suggested that Chinese medicine,western medicine and vitamin K have a certain effect on osteoporosis rats,especially Chinese medicine.However,calcium supplement has no obvious effect on osteoporosis.