碱性成纤维细胞生长因子共聚物缓释微球与兔跨区轴型皮瓣的成活

背景：研究表明使用生长因子直接或间接刺激新生血管形成可以促进皮瓣远端缺血部分的成活。目的：观察碱性成纤维细胞生长因子-聚乳酸-聚羟基乙酸共聚物缓释微球对家兔侧腹制作跨区轴型皮瓣成活的影响。方法：取24只健康家兔制作侧腹壁跨区轴型皮瓣,随机分组：实验组术前5d皮内注入碱性成纤维细胞生长因子-聚乳酸-聚羟基乙酸共聚物微球,对照组术前5d皮内注入碱性成纤维细胞生长因子＋空微球悬浊液,空白对照组术前5d皮内注入生理盐水。5d后掀起皮瓣原位缝合。结果与结论：①皮瓣成活率：实验组显著高于对照组和空白对照组（P〈0.01）,②皮瓣组织学变化：实验组新生血管增生明显,以毛细血管为主。③CD34＋免疫组织化学结果：实验组新生血管大量形成,平均血管数目高于对照组和空白对照组（P〈0.05）。表明术前5d局部注射碱性成纤维细胞生长因子-聚乳酸-聚羟基乙酸共聚物缓释球可促进皮瓣新生血管形成,增加皮瓣血运,促进皮瓣成活。

Effect of basic fibroblast growth factor copolymer sustained-release microspheres on the survival of cross-area flaps in rabbits

BACKGROUND： Studies have shown that directly or indirectly stimulating angiogenesis can promote the survival of distal ischemia in part. OBJECTIVE： To investigate the effect of basic fibroblast growth factor （bFGF）-poiy （lactic acid-glycolic acid） （PLGA）-sustained release microspheres on the survival of a cross-area flap on the lateral region of the rabbit abdomen METHODS： Twenty-four health rabbits were collected and the cross-area flap of rabbits was produced. Then the rabbits were randomly divided into three groups： experimental group, control group and blank control group. Five days before operation, rabbits in the experimental group were intradermally injected with bFGF-PLGA microspheres, and those in the control group were intradermally injected with bFGF＋empty microsphere suspension; while those in the blank control group were intradermally injected with normal saline. After 5 days, the skin flap was sutured. RESULTS AND CONCLUSION： （1）The skin flap survival rate of the experimental group was significantly higher than that of the control and blank control groups （P 〈 0.01）. （2）Histological changes of skin flap tissues： A large number of granulation tissue formed in the experimental group, and neovascutarization was obvious. （3）CD34＋ immunohistochemistry results： A large number of new vessels were found in the experimental group. In addition, the average number of blood vessels was higher in the experimental group than in the control group and blank control group （P 〈 0.05）. These findings suggest that the local injections of bFGF-PLGA sustained-release microspheres at 5 days preoperatively can promote skin flap neovascularization, increase the skin flap blood supply and promote skin flap survival.