姜黄素预处理及缺血后处理对大鼠肾脏缺血再灌注损伤的保护作用

背景：有研究发现姜黄素预处理、缺血后处理对大鼠肾脏缺血再灌注损伤具有保护作用。目的：建立大鼠肾脏缺血再灌注损伤模型,观察姜黄素预处理、缺血后处理、姜黄素预处理联合缺血后处理对大鼠肾脏缺血再灌注损伤的保护作用。方法：60只SD雄性大鼠随机均分为5组,假手术组、肾脏缺血再灌注模型组、姜黄素组、缺血后处理组以及联合处理组。肾脏再灌注24h后,取下腔静脉血测定肌酐和尿素氮。肾组织测定超氧化物岐化酶活性和丙二醛含量,并行苏木精-伊红染色观察各组肾组织病理学变化。结果与结论：与假手术组比较,其余各组肌酐、尿素氮均升高（P〈0.05）。与缺血再灌注模型组比较,姜黄素组、缺血后处理组和联合处理组的肌酐、尿素氮值在再灌注24h后均下降（P〈0.05）。与姜黄素组和缺血后处理组比较,联合处理组的肌酐更低（P〈0.05）。与假手术组比较,缺血再灌注模型组超氧化物歧化酶活性明显降低,丙二醛含量明显升高,差异有显著性意义（P〈0.05）。与缺血再灌注模型组比较,姜黄素组、缺血后处理组和联合处理组的超氧化物歧化酶活性升高,丙二醛含量降低,差异有显著性意义（P〈0.05）。与姜黄素组和缺血后处理组比较,联合处理组的超氧化物歧化酶活性升高,差异有显著性意义（P〈0.05）。与假手术组比较,缺血再灌注模型组肾小管损伤明显;与缺血再灌注模型组比较,姜黄素组、缺血后处理组和联合处理组肾小管损伤减轻明显,与姜黄素组和缺血后处理组比较,联合处理组损伤更轻。说明示姜黄素预处理和缺血后处理联合应用具有协同作用,可以更好的保护大鼠缺血再灌注损伤肾脏。

Protective effect of curcumin preconditioning and ischemic postconditioning against renal ischemia-reperfusion injury in rats

BACKGROUND： The studies have found that curcumin preconditioning and ischemic postconditioning have protective effect on renal ischemia-reperfusion injury. OBJECTIVE： To establish a model of renal ischemia-reperfusion injury and observe the protective effect of curcumin preconditioning, ischemic postconditioning and curcumin preconditioning combined with ischemic postconditioning on the renal ischemia-reperfusion injury of rats. METHODS： Sixty Sprague Dawley rats were randomly divided into five groups, namely sham-operation group, renal ischemia-reperfusion model group, curcumin preconditioning group, ischemic postconditioning group and curcumin preconditioning combined with ischemic postconditioning group （combination group）. After reperfusion for 24 hours, the inferior vena venous blood was extracted to determine the values of blood creatinine and urea nitrogen. The superoxide dismutase activity and malondialdehyde value were measured in renal tissue, and hematoxylin-eosin staining was used to observe the changes in renal structure. RESULTS AND CONCLUSION： Compared with sham-operation group, the values of blood creatinine and urea nitrogen in other four groups were significantly increased （P 0.05）. Compared with ischemia-reperfusion model group, the values of blood creatinine and urea nitrogen in the urcumin preconditioning group, ischemic postconditioning group and combination group were significantly decreased after reperfusion for 24 hours （P 0.05）. Compared with urcuminpreconditioning group and ischemic postconditioning group, the blood creatinine value of the combination group was significantly lower （P 0.05）. Compared with the sham-operation group, superoxide dismutase activity in the ischemia-reperfusion model group was decreased, while the value of malondialdehyde was increased significantly, the differences were statistically significant （P 0.05）. Compared with ischemia-reperfusion model group, urcumin preconditioning group, ischemic postconditioning group and combination group had higher superoxide dismutase activity, while the value of malondialdehyde was decreased; the differences were statistically significant （P 0.05）. Compared with urcumin preconditioning group and ischemic postconditioning group, superoxide dismutase activity of the combination group was significantly increased, and the difference was significant （P 0.05）. Compared with the sham-operation group, the renal tubular injury of ischemia-reperfusion injury model group was obvious; compared with ischemia-reperfusion model group, the urcumin preconditioning group, ischemic postconditioning group and the combination group had the significantly reduced tubular injury, and compared with urcumin preconditioning group and ischemic postconditioning group, the combination group had more significantly reduced tubular injury. Our study suggests that curcumin preconditioning combined with ischemic postconditioning has a synergistic effect; it can better protect the kidney from ischemia-reperfusion injury.