促红细胞生成素和甲状腺激素对K 562细胞铁代谢的影响

目的探讨重组人促红细胞生成素(rhEPO)和甲状腺激素(T3)对红白血病细胞K562铁代谢的影响及其可能的机制.方法参照骨髓铁染色的方法进行细胞铁染色,计算阳性细胞率,并通过流式细胞术观察转铁蛋白受体(TfR)的表达.结果 rhEPO与FeCl3共培养细胞时,可见铁染色阳性细胞率有明显上升,尤以rhEPO 5 U/ml组上升更为明显(P<0.01),且随培养时间延长,阳性率逐渐上升;不同浓度T3均使K562铁染色阳性细胞率明显上升,且以培养48 h组阳性率最高;不同浓度rhEPO培养细胞72 h均使K562细胞TfR表达明显增加(P<0.05),而不同浓度的T3对转铁蛋白受体的表达无明显作用.结论 rhEPO能增加K562细胞铁染色的阳性细胞率,其机制可能是由于增加TfR的表达使细胞大量摄入铁;而T3增加铁染色阳性细胞率的机制尚需进一步研究.

The Effect of Erythropoitin and Thyroid Hormone on K562 Iron Metabolism

Objective To explore the effect of recombinant human erythropoitin (rhEPO) and T 3 on the iron metabolism of K562 cells and its possible mechanism. Methods With the use of the bone marrow iron stain method, the positive stained cells rate was calculated. Flow cytometry was performed to detect the transferrin receptor (TfR) expression of K562 cells. Results rhEPO increased the positive cell rate of iron stain, wich was especially noticeable in the rhEPO 5 U/ml group ( P <0.01), and the effect was time dependant, i.e., the longer the incubating time, the higher the positive rate. T 3 also elevated the positive cell rate of iron stain significantly at different concentrations, and the the highest rate was seen when incubating K562 cells for 48 hours. The expression of TfR was increased significantly when incubating with rhEPO for 72 hours( P <0.05), but there was no marked change when incubating with T 3. Conclusion The possible mechanism by which rhEPO increased the positive cell rate of iron stain might be due to the enhancement of TfR expression and hence the increasein iron intake of K562 cells.