大鼠类风湿性关节炎药效学指标及检测方法

类风湿性关节炎（rheumatoid arthritis，RA）是一种以关节滑膜炎、破坏性关节病变为主要特征的慢性、进行性、侵袭性自身免疫性疾病，致畸、致残率极高且大多数患者反复发作。目前，改善临床症状的抗风湿药是治疗RA的主要手段，但由于传统抗风湿药的不良反应较大，而生物制剂价格昂贵，因而患者长期服用依从性差，不利于疾病控制。因此研制开发有效、不良反应小的抗风湿药物势在必行。研究并建立合理有效的RA动物模型是研制开发有效治疗药物和制订有效治疗措施的关键，而正确的检测也能准确地反映出药物的疗效。因此以Wistar大鼠为试验动物，对其进行免疫，从关节炎指数评定、血清学、病理学和影像学检测等方面进行综合评价，从而测评抗风湿药物药效。

Testing indexes and methods in pharmacodynamics experiment of rheumatoid arthritis with rats

Rheumatoid arthritis (RA) is a chronic, progressive, and aggressive autoimmune disease, the main characters of which include joint synovial inflammation and destructive arthropathy. RA is teratogenic and most of the patients are with high morbidity repeated attacks in the duration of symptoms. At present, medicine to improve the clinical symptoms of rheumatism is the main treatment for RA. But because of the side effects of traditional medicine and the cost of biological agents, patients have poor compliance in long-term use and there are difficulties in disease control. Therefore it is imperative to develop effective antirheumatic agents, with small side effects. To study and establish an effective rheumatoid arthritis animal model is the key to develop effective drugs and make effective treatment measures. Appropriate testing also could reflect the therapeutic efficacy of drugs accurately. So Wistar rats on the immune were used as test animals, and arthritis index evaluation, serologic, pathologic, and iconographic detection were carried out so as to evaluate the potency of antirheumatic agents.