| 大鼠脑组织中氨基酸类神经递质的rP-HPLC-UV柱前衍生定量分析 |
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| 引用本文： | 祁艳斌,张纬,刘德敏.大鼠脑组织中氨基酸类神经递质的rP-HPLC-UV柱前衍生定量分析[J].天津医科大学学报,1998(4). |
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| 作者姓名： | 祁艳斌 张纬 刘德敏 |
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| 作者单位： | 天津医科大学分析中心 |
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| 摘 要： | 目的：测定大鼠脑组织中纹状体、海马、皮质中氨基酸的含量，研究中枢神经系统及脑能量代谢和某些疾病之间的关系。方法：用反相高效液相色谱法测定脑组织中的８种游离氨基酸，以２，４－二硝基氟苯（ＤＮＦＢ）作为衍生剂，在ＵＶ３６０ｎｍ进行测定，以外标法进行计算。结果：在２０ｍｉｎ内分出８种氨基酸，分离完全，且纹状体氨基酸含量高于海马和皮质。结论：本方法简便，灵敏度高，分析时间短，适用于一般实验室。
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| 关 键 词： | 氨基酸 神经递质 HPLC |
QUANTITATIVE ANALYSIS OF AMINOACID NEUROTRANSMITTERS IN RAT BRAIN TISSUE BY RP HPLC PRE-COLUMN DERIVATIZATION |
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| Qi Yanbin,Zhang Wei,Liu Demin.QUANTITATIVE ANALYSIS OF AMINOACID NEUROTRANSMITTERS IN RAT BRAIN TISSUE BY RP HPLC PRE-COLUMN DERIVATIZATION[J].Journal of Tianjin Medical University,1998(4). |
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| Authors: | Qi Yanbin Zhang Wei Liu Demin |
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| Institution: | Analysis centre of Tianjin Medical University 300070 |
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| Abstract: | Objective:To determine the content of amino acid in corpus striatum, hippocampus and cortex of rat brain tissue, investigate the relationship of central nervous system, brain energy metabolism and certain diseases Methods: We analyzed 8 free amino acids by reversed phase high performance liquid chromatography The samples were derivated with DNFB ,detected with an UV detector at 360nm and calculated by outer criteria Results: This method can determine 8 amino acids in 20 minutes, and completely separate them We found that the content of amino acid in corpus striatum is higher than that of in hippocampus and cortex Conclusions: This method is simple, rapid and high sensible We believe it can be used successfully in common laboratory |
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| Keywords: | Amino acid Neurotransmitter HPLC |
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