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Stage and tissue specific differences in SjBMI1, a Polycomb protein in Schistosoma japonicum |
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| Authors: | Dong Hu Jing Wu Fengyu Hu Yabo Yang Chi Liang Jingfang Chen Lexun Wang Peng Wang Xiaoyun Wang Jin Xu Xuchu Hu Xinbing Yu |
| Affiliation: | 1. Department of Parasitology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, 510080, People’s Republic of China 2. Department of Biochemistry and Molecular Biology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, People’s Republic of China 3. Institute of Infectious Disease, Guangzhou No.8 People’s Hospital, Guangzhou, People’s Republic of China |
| Abstract: | Polycomb group protein BMI1, plays a central role in the stem cell pluripotency and development in metazoans. A gene encoding BMI1 homologue in the Schistosoma japonicum (SjBMI1) was cloned and identified. The deduced amino acid sequence shows high identity to the homologues from Schistosoma mansoni and Homo sapiens. Quantitative real time polymerase chain reaction (RT-PCR) and Western blot analysis revealed that the SjBMI1 is highly expressed in adult worms and eggs, not in cercariae. By immunofluorescent studies, SjBMI1 was localized to testes, ovaries of mixed sex infected adult worms, but not of single sex infected adult worms. The study reveals the SjBMI1 expression profile in developmental stages and localization characteristic and provides a clue that it may be associated with reproductive development of S. japonicum. |
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