川西吊石苣苔显微和化学特征研究

目的:为川西吊石苣苔的开发利用提供生药学和化学资料及深入研究奠定基础。方法:石蜡制片法、水合氯醛透化法和HPLC-UV法。结果:茎横切面上木栓层为一列排列紧密的细胞;大量石细胞存在于皮层;木质部较窄呈环状,髓部发达。叶横切面中上、下表皮各由1列类方形细胞组成,上表皮内方有2～3列大型上皮细胞;栅栏组织由2～3层长方形的薄壁细胞组成;主脉维管束为周韧型。粉末中大量梭形纤维存在,直径14.6～29.9μm;多为具缘纹孔导管;石细胞长方形,胞腔大,具孔沟;不定式气孔。川西吊石苣苔所含的苯乙醇苷类成分主要是阿克苷acteoside,caleolarioside B。结论:川西吊石苣苔的显微和化学特征可作为鉴定该植物的理论依据,也为该植物的深入研究奠定基础。

Pharmacognostical and chemical studies of Lysinotus wilsonii

Objective: To provide evidences for the pharmacognostical and chemical identification and the further development of Lysinotus wilsonii. Method : The paraffin section method was used for the microscopic identifications of stems and leaves. The slide with chloral hydrate was applied for the microscopic identifications of the powder of stems and leaves. The HPLC was used for the identification of the phenylpropanoids. Result : An obvious periderm consisted of a line of inseparable cells, lots of stone cells existed in the periderm and cortex, narrow ring of xylem and broad pith could be easily observed in the stem transaction of L. wilsonii. Epidermis was composed of one lines of parenchyma, three lines of epithelial cells in the side of above epidermis, palisade tissue was composed of 2-3 lines of square and thin cells and amphicribral vascular bundle in transaction of midrib also could be observed in the leaf transaction of L. wilsonii. Long xylem fibers, lots of pitted vessels, stone cells and anomocytic type stomas existed in the powder of L. wilsonii. Acteoside1 and caleolarioside B were detected in L. wilsonii. Conclusion : The pharmacognostic and chemical characteristics of L. wilsonii can be used for authentication of the plant.