应用Protein A亲和层析法制备及纯化R1JHL单克隆抗体

[目的]制备、纯化N-甲基D-门冬氨酸受体（NMDAR，NR）主亚基RIJHL单克隆抗体。[方法]NR1杂交瘤细胞腹腔注射BalB／C小鼠制备抗体，Protein A-Sepharose CL-4B亲和层析纯化单抗，Western-blot鉴定该单抗特异性。[结果]纯化后单抗识别大鼠脑组织膜蛋白中约115kD大小的单一条带。[结论]制备、纯化了具有高度特异性的抗NR1单克隆抗体，为进一步研究NMDA受体提供了工具。

Preparation and purification of mouse monoclonal antibody against NMDAR1 by ProteinA-Sepharose CL-4B affinity chromatography

[Objective] To prepare and purify the monoclonal antibody against NMDAR1.[Methods] NR1 Hybridoma cells were injected into the abdominal cavity of BalB/C mice to prepare the monoclonal antibody against NMDAR1.The antibodies were purified by ProteinA-Sepharose CL-4B affinity chromatography and its specificity were evaluted by Western blot.[Results] Western blot comfired that the antibodies specifically recognized the NR1 membrane protein extracted from rat brain tissues.[Conclusion ] The purified antibodies c...