miR-21、miR-155在乳腺癌患者血液与肿瘤组织中表达水平的相关性研究

目的 对健康女性血清及乳腺癌患者血清中miR-21和miR-155表达水平进行实时定量PCR检测,同肿瘤患者癌组织与对应癌旁组织的miR-21和miR-155表达水平的变化趋势相比较,以评价血清中miR-21和miR-155在乳腺癌早期诊断中的潜在价值.方法 收集健康女性血液和患者血液与患者癌组织和癌旁组织,利用实时荧光定量PCR对提取的RNA miR-21和miR-155进行相对定量检测,得到miR-21和miR-155在血清中变化趋势与在组织中变化趋势的相关性.结果 miR-21和miR-155在肿瘤组织的表达水平明显高于其在癌旁组织的表达水平,miR-21和miR-155在乳腺癌患者血清中的表达水平也要高于其在健康体检者血清中的表达水平.血清中miR-21和miR-155表达水平变化趋势与组织中miR-21和miR-155表达水平变化趋势一致.结论 血清中miR-21和miR-155能反映乳腺癌的发生,miR-21和miR-155有可能作为乳腺癌早期诊断的肿瘤标志物.

Correlation study of the expression of miR-21 and miR-155 in the blood and tumor tissue of patients with breast cancer

Objective To evaluate the expression levels of miR-21 and miR-155 in the serum of breast cancer patients and healthy women by real-time quantitative PCR,compared with the variation trend of the expression level of miR-21 and miR-155 in tumor tissue samples.Methods The samples were collected from the intraoperative tumor tissue,adjacent tissue,and the peripheral blood of patients and from the peripheral blood of healthy women.The miR-21 and miR-155 obtained were detected by real time quantitative PCR.Then the relationship between the trend of miR-21 and miR-155 in serum and in tumor tissues was analyzed.Results The expression levels of miR-21and miR-155 in adjacent tissues of breast cancer patients were significantly lower than those in tumor tissues.The expression levels of miR-21 and miR-155 in the serum of breast cancer patients were significantly higher than those of healthy women.Conclusion The levels of serum miR-21 and miR-155 can be used as biomarkers for the early diagnosis of breast cancer.