川芎嗪注射液抑制子宫内膜异位症微血管密度的实验研究

目的：观察川芎嗪注射液（TMP）对大鼠子宫内膜异位症微血管密度的影响和抗血管形成作用。方法：建立Wistar大鼠子宫内膜异位症动物模型,4周后将建模成功的大鼠随机分为对照组、ip TMP 50,100,200 mg/（kg.d）连续给药15d,SP免疫组化法测定在位内膜和异位病灶组织中MVD的表达。结果：TMP治疗EMs模型大鼠4周后异位组织中MVD的表达明显低于对照组,差异有显著意义（P〈0.05）,而在位内膜中MVD的表达与对照组相比,差异无显著意义（P〉0.05）;TMP可剂量依赖性的抑制EMs异位组织的增生（P〈0.05）。结论：TMP对子宫内膜异位症大鼠异位组织中MVD的表达和新生血管形成均有抑制作用,作用呈剂量依赖性。TMP有望成为子宫内膜异位症抗血管形成治疗的新药。

Inhibitory effect of TMP on microvessel density in rat endometriosis

Objective：To observe the effect of TMP on micro-vessel density（MVD） and antiangiogenesis in rat endometriosis.Methods：Wistar rat endometriosis models were established,after 4 weeks,the successful endometriosis rats were divided into control group,TMP50mg·kg-1·d-1group,TMP100mg·kg-1·d-1group and TMP200 mg·kg-1·d-1group and the rats were given successive intragastric administration for 15 days.Ectopic endometrium graft volume was observed and MVD was determined by immunostaining for CD34.Results：MVD in endometriosis was decreased remarkably after 4-week treatment with TMP（P0.05）,with was no significant difference.TMP could significantly inhibit proliferation of ectopic endometrium in a dose-dependent manner.Conclusion：TMP has an inhibitory effect on the MVD expression and angiogenesis in rat endometriosis in a dose-dependent manner.TMP may become a new drug of antiangiogenesis in endometriosis.