| 用多重PCR方法鉴别不同卡介苗菌株 |
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| 引用本文： | 刘菊,刘少祥,潘海龙,严君喜. 用多重PCR方法鉴别不同卡介苗菌株[J]. 中国生化药物杂志, 2012, 33(4): 447-449 |
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| 作者姓名： | 刘菊 刘少祥 潘海龙 严君喜 |
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| 作者单位： | 成都生物制品研究所,四川成都,610023 |
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| 摘 要： | 目的用多重PCR方法鉴别不同卡介苗菌株。方法根据卡介苗DNA的6个目的区域的特点设计引物,进行多重PCR扩增,然后电泳检测分析。结果多重PCR方法能够鉴别不同卡介苗亚株。结论应用多重PCR方法鉴别卡介苗菌株具有专一性好、重复性高、快速简便等优点。
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| 关 键 词： | 卡介苗 鉴别 多重PCR |
Preliminary study on the identification of substrains of BCG vaccine using multiplex PCR |
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| LIU Ju , LIU Shao-xiang , PAN Hai-long , YAN Jun-xi. Preliminary study on the identification of substrains of BCG vaccine using multiplex PCR[J]. Chinese Journal of Biochemical Pharmaceutics, 2012, 33(4): 447-449 |
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| Authors: | LIU Ju LIU Shao-xiang PAN Hai-long YAN Jun-xi |
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| Affiliation: | (Chengdu Institute of Biological Products,Chengdu 610023,China) |
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| Abstract: | Purpose Using a multiplex PCR method to identify BCG substrains.Methods A multiplex PCR was performed with 13 primers against six different targets and the amplification products were seperated on an agarose gel.Results BCG substrains can be distinguished from each other by using multiplex PCR.Conclusion This multiplex PCR method can be used as a tool for the rapid,specific and reproducible identification of BCG. |
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| Keywords: | BCG identification multiplex PCR |
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