| 高效液相色谱法测定两种岩陀中岩白菜素的含量 |
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| 引用本文： | 彭朝霞,杨朝莲,熊然英,晏祥杰. 高效液相色谱法测定两种岩陀中岩白菜素的含量[J]. 中国生化药物杂志, 2012, 33(4): 419-421 |
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| 作者姓名： | 彭朝霞 杨朝莲 熊然英 晏祥杰 |
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| 作者单位： | 六盘水市药品检验所,贵州六盘水,553001 |
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| 摘 要： | 目的采用高效液相色谱法测定岩陀中岩白菜素的含量。方法色谱柱为Hypersil C18柱(4.6 mm×250mm,5μm),流动相为乙腈-水(10∶90),检测波长为274 nm,柱温25℃。结果岩白菜素的线性范围为0.049 21～3.396 8μg,r=0.999 8,加样回收率为99.8%,RSD为1.18%(n=9)。结论本文所建立的方法简单、准确、可靠、重复性好,可用于岩陀中岩白菜素的含量测定。
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| 关 键 词： | 岩陀 岩白菜素 含量测定 |
HPLC determination of bergeninum in two kinds of Rhizoma Rodgersiae Sambucifoliae |
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| PENG Zhao-xia , YANG Chao-lian , XIONG Ran-ying , YAN Xiang-jie. HPLC determination of bergeninum in two kinds of Rhizoma Rodgersiae Sambucifoliae[J]. Chinese Journal of Biochemical Pharmaceutics, 2012, 33(4): 419-421 |
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| Authors: | PENG Zhao-xia YANG Chao-lian XIONG Ran-ying YAN Xiang-jie |
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| Affiliation: | (Liupanshui Institute for Drug Control,Liupanshui 553001,China) |
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| Abstract: | Purpose To establish a method for determination of bergeninum in Rhizoma Rodgersiae Sambucifoliae by HPLC.Methods The HPCL system was conducted as follows:Hypersil C18 column(4.6 mm ×250 mm,5 μm),acetonitrile-water(10∶ 90)mixture as a mobile phase,detection wavelength at 274 nm,1.0 mL/min of flow rate and column temperature at 25 ℃.Results The linear range of bergeninum was 0.049 21-3.396 8 μg,r=0.999 8,the recovery was 99.8% and RSD=1.18(n=9).Conclusion The method is simple,accurate of higher sensitivity and reproducibe.This method can be used for the determination of bergeninum in Rhizoma Rodgersiae Sambucifoliae. |
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| Keywords: | Rhizoma Rodgersiae Sambucifoliae bergeninum content determination |
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