| UPLC-Q-TOF/MS分析埃博霉素B |
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| 引用本文： | 王堃,王延亮,卢育新,丁小军,程晓晨,张庆林. UPLC-Q-TOF/MS分析埃博霉素B[J]. 中国生化药物杂志, 2012, 33(4): 392-394,398 |
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| 作者姓名： | 王堃 王延亮 卢育新 丁小军 程晓晨 张庆林 |
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| 作者单位： | 1. 安徽医科大学研究生院,安徽合肥230032;北京放射与辐射医学研究所,北京100850
2. 北京放射与辐射医学研究所,北京,100850 |
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| 摘 要： | 目的采用UPLC-Q-TOF/MS(超高效液相色谱联合飞行时间串联质谱)对埃博霉素B纯化产品及黏细菌发酵液提取物进行分析。方法运用ACQUITY HSS T3超高效液相色谱柱(2.1 mm×50 mm,1.8μm),以甲醇-0.1%甲酸溶液为流动相梯度洗脱;质谱使用ESI离子源,负离子模式下采集数据。结果运用UPLC-Q-TOF/MS方法可以检测到埃博霉素B纯化产品中的微量杂质以及发酵液提取物中主要产物。结论 UPLC-Q-TOF/MS方法操作简单、快捷,可作为埃博霉素B的检测方法。
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| 关 键 词： | 黏细菌 埃博霉素B UPLC-Q-TOF/MS |
Analysis of Epothilone B by UPLC-Q-TOF/MS |
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| WANG Kun , WANG Yan-liang , LU Yu-xin , DING Xiao-jun , CHENG Xiao-chen , ZHANG Qing-lin. Analysis of Epothilone B by UPLC-Q-TOF/MS[J]. Chinese Journal of Biochemical Pharmaceutics, 2012, 33(4): 392-394,398 |
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| Authors: | WANG Kun WANG Yan-liang LU Yu-xin DING Xiao-jun CHENG Xiao-chen ZHANG Qing-lin |
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| Affiliation: | 1.Graduate School,Anhui Medical University,Hefei 230032,China;2.Beijing Institute of Radiation Medicine,Beijing 100850,China) |
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| Abstract: | Purpose To analyze Epothilone B in purified products and the fermentation broth of Sorangium cellulosum by ultra performance liquid chromatography coupled with time-of-flight mass spectrometry(UPLC-Q-TOF/MS).Methods Epothilone B was determined with an ACQUITY UPLC HSS T3(2.1×50 mm,1.8 μm)by gradient elution using methanol and 0.1% formic acid as mobile phase.ESI source was used and data were acquired in negative mode.Results Epothilone B and Epothilone A were separated and identified in the Epothilone B purified products and the fermentation broth extracts.Conclusion The UPLC-Q-TOF/MS method is simple and fast for the rapid determination of Epothilone B. |
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| Keywords: | myxobacterium Epothilone B UPLC-Q-TOF/MS |
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