苏拉明对体外培养的人视网膜色素上皮细胞增殖的影响

目的 观察苏拉明(suramin)对体外培养的人视网膜色素上皮细胞(retinalpigment epithelial cells)生长、增殖的影响。方法 以300mg·L~(-1)苏拉明作用于培养的视网膜色素上皮细胞,于不同的时间点行细胞计数,绘制生长曲线,台盼蓝染色判定细胞的活性;将不同浓度的苏拉明(0、100、200、300、400mg·L~(-1))加入RPE细胞培养液,72h后用四甲基唑盐(MTT)比色法测吸光值A,并计算不同浓度条件下的细胞增殖抑制率;流式细胞技术检测细胞周期变化。结果 苏拉明在所设浓度内均抑制RPE细胞增殖,并存在时间-效应及剂量-效应关系,最大增殖抑制率约78％,72h时IC_(50)为272mg·L~(-1)。FCM示suramin使G_2/M期细胞增加了14.2％。结论 苏拉明能抑制体外培养的人视网膜色素上皮细胞增殖,可作为防治增生性玻璃体视网膜病变(proliferative vitreoretinopathy,PVR)的候选药物作进一步研究。

Effects of suramin on proliferation of human retinal pigment epithelial cells in vitro

Objective To investigate the effects of suramin on proliferation of cultured human retinal pigment epithelial cells (HRPECs). Methods Cultured HRPECs were treated with 300mg L-1 suramin for different times (24 ~ 168h), the cell growth curve was drawn according to cell counting and the viability of cells was determined by trypan blue exclusion; different concentrations of suramin (0, 100,200, 300, 400mg L-1) were added to HRPECs respectively, and inhibition rate of retinal pigment epithelial (RPE) cells was measured by tetrazolium (MTT) colorimetric assay; flow cytometry(FCM) analysis was used to examine RPE cells cycles. Results Suramin could inhibit the proliferation of HRPECs in a dose-dependent and time-dependent manner. The maximal inhibition rate of RPE cells by suramin was about 78% , and IC50 at 72h was 272mg L-1. FCM revealed that cells in G2/M phase increased 14. 2% compared to control group. Conclusion Suramin can effectively inhibit HRPECs growth in vitro, and it might be a new candidate drug of preventing for patients with proliferative vitreoretinopathy.