Standardization of chaga tincture and befungin

Methods for standardizing the formulations Chaga Tincture and Befungin were developed, consisting of extraction of the non-phenol fraction predominantly containing tetracyclic triterpenes, including lanosterol and ergosterol, followed by spectrophotometry of colored complexes with vanillin in acidic conditions. The contents of tetracyclic triterpenes expressed as lanosterol ranged from 0.01% to 0.035% in Chaga Tincture and from 0.01% to 0.02% in Befungin.