| 产超广谱β-内酰胺酶的肺炎克雷伯菌相关耐药基因研究 |
| |
| 引用本文： | 史伟峰,王玉月,李美忠,周军.产超广谱β-内酰胺酶的肺炎克雷伯菌相关耐药基因研究[J].上海医学检验杂志,2009(3). |
| |
| 作者姓名： | 史伟峰 王玉月 李美忠 周军 |
| |
| 作者单位： | 苏州大学附属第三医院检验科; |
| |
| 摘 要： | 目的了解常州地区肺炎克雷伯菌耐药状况及β-内酰胺类耐药基因。方法用琼脂稀释法检测氨苄西林、哌拉西林、哌拉西林-他唑巴坦、头孢噻肟、头孢他啶、头孢吡肟、氨曲南和亚胺培南对肺炎克雷伯菌的最低抑菌浓度(MIC)。采用聚合酶链反应(PCR)检测产超广谱β-内酰胺酶(ESBLs)肺炎克雷伯菌16种相关耐药基因,并用DNA测序仪测序。结果120株肺炎克雷伯菌ESBLs阳性率为44.2%(53株)。从53株肺炎克雷伯菌中检出TEM、SHV、CTX-M-1群、CTX-M-9群、OXA-1群、LEN、OKP和DHA 8种β-内酰胺酶基因,阳性率分别为75.5%、22.6%、18.9%、7.5%、11.3%、5.7%、3.8%和41.5%,其中2株菌同时检出6种β-内酰胺酶基因。基因测序证实为TEM-1、TEM-11、SHV-13、SHV-28、CTX-M-22、CTX-M-55、OXA-1和OKP-6等。结论本地区肺炎克雷伯菌已携带多种β-内酰胺酶耐药基因,是其对β-内酰胺类抗菌药物耐药的重要原因之一。
|
| 关 键 词： | 肺炎克雷伯菌 β-内酰胺酶 耐药基因 |
Study on the drug-resistant genes associated with extended-spectrum beta-lactamases in Klebsiella pneumoniae |
| |
| SHI Weifeng,WANG Yuyue,LI Meizhong,ZHOU Jun..Study on the drug-resistant genes associated with extended-spectrum beta-lactamases in Klebsiella pneumoniae[J].Shanghai Journal of Medical Laboratory Sciences,2009(3). |
| |
| Authors: | SHI Weifeng WANG Yuyue LI Meizhong ZHOU Jun |
| |
| Institution: | Department of Clinical Laboratory;the Third Affiliated Hospital of Suzhou University;Jiangsu Changzhou 213003;China |
| |
| Abstract: | Objective To investigate the resistance to antimicrobials and resistant genes associated with beta-lactams in Klebsiella pneumoniae(K.pneumoniae) isolated from Changzhou district.Methods Minimum inhibitory concentrations(MIC) of ampicillin,piperacillin,piperacillin-tazobactam,cefotaxime,ceftazidime,cefepime,aztreonam and imipenem were determined by agar dilution method.Sixteen resistant genes of K.pneumoniae producing extended-spectrum beta-lactamases(ESBLs) were detected by polymerase chain reaction(PCR) a... |
| |
| Keywords: | Klebsiella pneumoniae Beta-lactamase Resistant gene |
| 本文献已被 CNKI 等数据库收录！ |
