| EGF促进RPE细胞毒蕈碱受体诱导的磷酸肌醇形成：受体间交互作用分析 |
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| 引用本文： | 刘宁朴,Osborne NN,张惠蓉. EGF促进RPE细胞毒蕈碱受体诱导的磷酸肌醇形成：受体间交互作用分析[J]. 中华眼底病杂志, 1994, 10(4): 220-222 |
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| 作者姓名： | 刘宁朴 Osborne NN 张惠蓉 |
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| 作者单位： | 北京医科大学第三医院眼科 |
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| 基金项目： | 国家自然科学基金，国家教委科研基金 |
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| 摘 要： | 对培养人眼视网膜色素上皮（RPE）细胞磷酸肌醇（InsPs）水平的分析表明，Carbachol显著刺激RPE细胞InsPs水平的升高，且该效应可被阿托品所阻断，表明在人眼RPE细胞存在毒蕈碱受体。去甲肾上腺素、5-羟色胺、表皮生长因子（EGF）、异丙肾上腺素和NECA对InsPs基础水平无明显影响。异丙肾上腺素和NECA对Carbachol刺激InsPs的效应也无明显影响；但EGF显著促进Carbacol刺激人眼RPE细胞InsPs水平升高的效应。提示在人眼RPE细胞EGF与毒蕈碱受体之间存在有受体间交互作用。（中华眼底病杂志，1994，10：220-222）
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| 关 键 词： | 视网膜色素上皮，细胞培养，表皮生长因子，毒章碱受体，磷酸肌醇 |
| 收稿时间： | 1993-10-23 |
| 修稿时间： | 1994-08-18 |
EGF POTENTIATES THE MUSCARINIC RECEPTOR-INDUCED STIMULATION OF INOSITOL PHOSPHATES IN RPE CELLS:ANALYSIS OF RECEPTOR INTERACTION |
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| Liu Ningpu,Osborne NN,Zhang Hulrong. EGF POTENTIATES THE MUSCARINIC RECEPTOR-INDUCED STIMULATION OF INOSITOL PHOSPHATES IN RPE CELLS:ANALYSIS OF RECEPTOR INTERACTION[J]. Chinese Journal of Ocular Fundus Diseases, 1994, 10(4): 220-222 |
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| Authors: | Liu Ningpu Osborne NN Zhang Hulrong |
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| Affiliation: | Dept.Ophthalmology,Third Hospital,Beijing Medical University,Beijing 100083 |
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| Abstract: | n this study iwe show that carbachol stimulates the accumulation of inositol phosphates(insps)in human retinal pigment epithelium (RPE)cells and atropine blocks the carbachol-induced effect,suggesting the existence of muscarinic acetylcholine receptors in human RPEcells. In contrast . noradrenaline. serotonin. epidermal growth factor (EGF) , isoproterenol. andNECA ( 5-[N-ethyl]-carboxamido-adenosine ) do not influence the basal levels of insps.Moreover. isoproterenol and NECA do not affect the carbachol-elevated levels of insPs.EGF.however ,does potentiate the carbachol stimulated elevation of Insps in a dose-depen-dent rnanner ,suggesting an interaction between EGF and muscarinic receptors in cultured hu-nian RPE cells. |
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| Keywords: | retinal pigment epithelium cell culture epidermal growth factormuscarinic receptor inositol phosphates |
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