人蜕膜移植建立NOD-SCID鼠子宫内膜异位症模型

目的 探讨采用蜕膜建立人子宫内膜异位症移植动物模型的可行性。方法 将早孕期蜕膜组织种植于NOD-SCID鼠腹部皮下，随机分成4组，分别在2、4、6、8周，取出其皮下移植物送病理检查，并用免疫组织化学的方法观察其形态学、增殖活性方面有无改变。结果 16只小鼠均存活，有15只小鼠皮下病灶经HE染色可见明显的子宫内膜腺体和间质，成功率为93．75％。腺上皮细胞角蛋白、ki67染色均阳性，基质细胞波形蛋白染色阳性，8周移植物中一些基质细胞减少区域的腺上皮细胞波形蛋白染色阳性。结论 采用人蜕膜建立NOD-SCID小鼠子宫内膜异位症模型成功率高，模型形状显著且稳定。

Establishment of animal model of endometriosis with decidua

Objective To establish the implanted animal model of human endometriosis with deciduas. Methods The decidua of early pregnant was implanted subcutaneously in the abdomen of NOD-SCID mice.The mice were randomly divided into four groups. The implant was saved for pathology examination at 2nd,4th,6th,8th weeks. Results Successful subcutaneous implantation was observed in 15 of 16 (93.75%) NOD-SCID mice. Haematoxylin-eosin staining of implanting lesions demonstrated the presence of endometrial glandular tissue in a background of stromal cells. The glandular epithelium exhibited positive cytokeratin and ki67 staining.The stromal cells exhibited positive vimentin staining. The glandular epithelium exhibited positive vimentin staining in combination with a decrease of surrounding stromal cells at 8th week. Conclusion The establishment of the animal model of endometriosis with deciduas is successful.