子宫颈鳞癌中微血管密度与CD44v6表达及其临床意义

目的　探讨子宫颈鳞状上皮细胞癌间质中微血管密度 (MVD)和CD44v6表达及其与临床病理的关系。方法　应用CD44v6和CD3 4 单克隆抗体 ,采用微波 -LSAB免疫组化染色方法对 5 0例宫颈鳞癌进行免疫组化染色 ,并取 10例正常宫颈组织作对照。结果　有淋巴结转移组宫颈鳞癌MVD、CD44v6与无淋巴结转移组、正常对照组之间比较 ,均有非常显著的差异性 (P <0 0 1) ;浸润癌中CD44v6与原位癌、早期浸润癌比较 ,均有显著差异 (P <0 0 5 )。结论　MVD和CD44v6表达水平与宫颈鳞癌浸润转移密切相关 ,可作为预测宫颈鳞癌病人预后和转移的生物学指标

Microvessel Density and CD44v6 Expression in Uterine Cervical Squamous Carcinoma and Their Clinical Significance

Objective To analyse the microvascular density(MVD) and CD44v6 expression in uterine cervical squamous carcinoma to evaluate their clinical significance. Methods Immunohistochemical staining (LSAB method) by using antibodies against CD 34 and CD44v6 was performed on archival specimens of 50 cases of human uterine cervical squamous carcinoma and 10 cases of normal cervical tissue. Results MVD was greater in metastatic tumors than that in nonmetastatic tumors and normal control. The expression of CD44v6 was higher in metastatic tumors than that in nonmetastatic tumors and normal control. MVD and CD44v6 expressions were significantly related to lymph node metastasis(P<0 01).The expression of CD44v6 in the invasive carcinoma increased compared with carcinoma in situ and early invasive carcinoma(P<0 05). Conclusions MVD and the CDv44v6 expression levels were associated with invasion and metastasis of uterine cervical cancer. MVD and CD44v6 positive expression may be used as a hopeful marker for predicting the prognosis and metastasis of patients with uterine cervical cancer.