免疫毒素BDI—1—PEA的构建及其活性测定

目的 构建新型免疫毒素BDI-1-PEA，并进行抗入膀胱肿瘤活性的初步研究测定。方法 应用异型双功能连接剂N-琥珀酰胺酯3-（2-吡啶基二硫）丙酸（SPDP）将抗人膀胱肿瘤单克隆抗体（BDI-1）与铜绿假单胞菌外毒素（PEA）交联，制备出新型免疫毒素BDI-1-PEA。应用四甲基偶氮唑盐（MTT）法初步检测其对人膀胱肿瘤细胞系E-J的选择性杀伤活性。结果 BDI-1-PEA结合物具有高效靶向杀伤膀胱肿瘤细胞的能力，优于游离PEA或BDI-1的混合物。结论 新型免疫毒素BDI-1-PEA有较高的选择性抗人膀胱肿瘤细胞的活性，有较高的研究的应用价值。

The construction of immunotoxin BDI- 1 - PEA and the test for its cytotoxicity against human bladder carcinoma in vitro

Objective To construct the immunotoxin BDI-1-PEA and study its cytotoxicity against human bladder carcinoma in vitro. Methods The immunotoxin BDI-1-PEA was prepared by conjugating pseudomonas exotoxin A (PEA) with monoclonal anti-bladder cancer antibody BDI-1 by using N-succinimidyl 3-(2-pyridyldithio) propionate (SPDP). Results Study in vitro indicated that BDI-1-PEA showed stronger selective cytotoxicity against human bladder carcinoma cell line E-J than free PEA or the mixture of BDI-1 and PEA. Conclusion BDI-1-PEA conjugate is an effective immunotoxin against human bladder carcinoma and is a promising candidate for further clinical study.