| 紫外分光光度法测定两面针中总生物碱的含量 |
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| 引用本文： | 章伟,刘韶,何桂霞. 紫外分光光度法测定两面针中总生物碱的含量[J]. 中国中医药信息杂志, 2008, 15(1): 50-51 |
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| 作者姓名： | 章伟 刘韶 何桂霞 |
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| 作者单位： | 1. 湖南中医药大学药学院,湖南,长沙,410004
2. 中南大学湘雅医院药剂科,湖南,长沙,410008 |
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| 摘 要： | 目的建立紫外分光光度法测定两面针中总生物碱含量的方法。方法采用紫外分光光度法，以氯化两面针碱为对照，在329nm波长处进行含量测定。结果线性范围为3～8μg／mL，回归方程：Y=97．746X-0．0255，r=0．9991（n=6）。氯化两面针碱的平均回收率为99．5％，RSD=2．32％（n=5）。结论方法简便、提取完全、重现性好、结果准确。
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| 关 键 词： | 两面针 生物碱 含量测定 紫外分光光度法 |
| 文章编号： | 1005-5304（2008）01-0050-02 |
| 收稿时间： | 2007-04-16 |
| 修稿时间： | 2007-04-16 |
Determination of Alkaloids in Zanthoxylum by Ultraviolet Spectrophotometry |
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| ZHANG Wei,LIU Shao,HE Gui-xia. Determination of Alkaloids in Zanthoxylum by Ultraviolet Spectrophotometry[J]. Chinese Journal of Information on Traditional Chinese Medicine, 2008, 15(1): 50-51 |
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| Authors: | ZHANG Wei LIU Shao HE Gui-xia |
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| Affiliation: | ZHANG Wei, LIU Shao, HE Gui-xia ( 1.Hunan University of TCM, Changsha 410004, China; 2.Xiangya Hospital of South China University, Changsa 410008, China) |
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| Abstract: | Objective To establish a simple method for determination the Alkaloids contents from Zanthoxylum nitidum. Methods Adopting nitidunechloride as reference, alkaloids in Zanthoxylum nitidum were by ultraviolet spectrophotometry at 329 nm. Results The liner arrange was 3-8 μg/mL, regression equation.. Y=97.75X-0.025 5, r =0.999 1 （n =6）. The mean recovery of Nitidunechloride was 99.46%, RSD=0.93% （n =5）. Conclusion The method performed is accurate and simple. The reproducibility and rate of extraction are also desirable. |
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| Keywords: | Zanthoxylum nitidum Alkaloids content determination ultraviolet spectr-ophometry |
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