人核迁移蛋C刺激人造血干细胞增殖分化为巨核细胞研究

目的:研究人核迁移蛋C(hNUDC)促进人脐血来源的CD34 细胞增殖、分化为巨核细胞的作用.方法:使用Dynal CD34体外分离系统收集人脐血CD34 细胞, 无血清甲基纤维素半固体法体外培养CD34 细胞12 d后, 显微镜下观察hNUDC对CD34 细胞分化增殖为小、中、大巨核细胞集落的形态、数目的影响;无血清液体培养体系培养CD34 细胞10 d后, 流式细胞术检测hNUDC对CD34 细胞分化增殖为CD41 细胞的表达率及其DNA倍性的影响.结果:hNUDC能够明显促进CD34 细胞分化增殖形成中小型CFU-MK集落, 可显著增加巨核细胞表面标志物CD41 的表达, CD41 细胞中DNA倍性显著地高于血小板生成素.结论:hNUDC对促进造血干细胞增殖和分化为巨核细胞具有重要作用.

hNUDC promotes proliferation and differentiation of megakaryocytopoiesis on human CD34+ cells

AIM: aTo study the effect of human nuclear distribution C èhNUDCé on human megakaryocyte proliferation and differentiation from cord blood CD34(+) cells in vitro. METHODS: aHuman CD34(+) cells were isolated using the Dynal CD34 Progenitor Cell Selection System from umbilical cord blood. The CD34(+) cells were then cultured in serum free methylcellulose semi-solid media, the morphologic aspects and number of small, medium and large CFU-MK colonies were observed and scored on the day12 by microscopy analysis. The CD34(+) cells were cultured in serum free liquid media, cells were removed on day 10 and formation of CD41(+) in human megakaryocyte and its DNA polyploidization of nuclear were analyzed on a FACsort flowcytometer. RESULTS: ahNUDC supported the formation of small and medium CFU-MK colony in serum free semi-solid media. Furthermore, hNUDC induced a remarkable increase in expression of the megakaryocyte cell surface marker CD41(+) and stimulated the CD41(+) DNA polyploidization more effectively than TPO. CONCLUSION: hNUDC may play an important role in megakaryocyte proliferation and differentiation.