Gallbladder Inflammation is Associated with Increase in Mucin Expression and Pigmented Stone Formation |
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Authors: | Alexander Vilkin Israel Nudelman Sara Morgenstern Alex Geller Yosefa Bar Dayan Zohar Levi Galina Rodionov Britta Hardy Fred Konikoff Diana Gobbic Yaron Niv |
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Institution: | (1) Department of Gastroenterology, Rabin Medical Center, Beilinson Campus, Tel Aviv University, Petach Tiqwa, 49100, Israel;(2) Department of Surgery, Rabin Medical Center, Beilinson Campus, Petach Tiqwa, 49100, Israel;(3) Department of Pathology, Rabin Medical Center, Beilinson Campus, Tel Aviv University, Petach Tiqwa, 49100, Israel;(4) Felsenstein Medical Research Center, Rabin Medical Center, Beilinson Campus, Tel Aviv University, Petach Tiqwa, 49100, Israel;(5) Minerva Center for Cholesterol Gallstones and Lipid Metabolism in the Liver, Tel Aviv University, Tel Aviv, Israel |
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Abstract: | Mucin is a high molecular weight glycoprotein that plays an important role in protecting the gallbladder epithelium from the
detergent effect of bile. However, it also participates in gallstone formation. There is little information about a possible
relationship between gallbladder inflammation and mucin expression or gallbladder stones’ characteristics. The aims of this
study were to investigate stone characteristics and patterns of mucin expression in the gallbladder epithelium and bile of
gallstone patients, in relation to inflammation. Gallbladder bile and tissue samples from 21 patients were obtained at surgery.
Mucin content was evaluated by gel filtration on a Sepharose CL-4B column. Dot blot for bile mucin apoproteins and immunohistochemistry
staining for gallbladder mucosal mucin apoproteins were performed with antibodies to MUC2, MUC3, MUC5AC, MUC5B and MUC6. Staining
intensity score (0–3) was used for assessment of antigen expression and the level of inflammation. Gallstone cholesterol content
was determined in 16 patients. MUC 5AC and MUC 5B were demonstrated in 95.4 and 100% of gallbladder bile samples, respectively.
Immunohistochemistry staining with antibodies to MUC 2, MUC 3, MUC 5AC, MUC 5B and MUC 6 were positive in 0, 100, 85.7, 100
and 95.4% of the gallbladder mucosal samples, respectively. Pigmented brown stones were associated with a higher level of
gallbladder inflammation. Mucin species expressed in gallbladder epithelium are MUC3, MUC5AC, MUC5B and MUC6. MUC5AC and MUC5B
are secreted into bile. Inflammation of the gallbladder is accompanied by a higher level of MUC5AC expression and is associated
with pigmented brown stones. |
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Keywords: | Mucin Gallbladder Bile Inflammation |
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