高效液相色谱法测定抗衰合剂中丹参素钠的含量

目的：建立高效液相色谱法测定抗衰合剂中丹参素钠的含量。方法：色谱柱：Hypersil BDS柱（250mm×4．6mm，5μm）；流动相：甲醇-水-冰醋酸（8：91：1）；流速：1．0ml·min^-1；检测波长：281nm；柱温：30℃；灵敏度：0．10AUFS。结果：丹参素钠在6．21—198．72μg·ml^-1浓度范围内线性关系良好，r=0．9999，平均回收率为97．6％（RSD=1．55％），98．3％（RSD=1．19％），96．5％（RSD=1．32％）。结论：该方法操作简便，快捷，可靠性高，准确性和重复性好，其他组分对测定无干扰，可作为抗衰合荆的质量控制方法。

Determination of Sodium Danshensu in Kangshuai Mixtura by HPLC

Objective： To establish a method for determining the content of sodium danshensu in kangshuai mixtura by HPLC. Method： Sodium Danshensu was assayed with Hypersil BDS column（ 250mm×4.6mm,5μm）. The mobile phase was methanol-water- anetic acid （8：91：1 ） ,the flow rate was 1.0ml· min^-1, the detection wavelength was 281nm and column temperature was 30℃. Result： The calibration curve was linear in the range of 6.21 -198. 72μg·ml^-1 with good linearity （r = 0. 999 9 ）. The average recovery was 97.6% （RSD = 1.55% ） ,98. 3% （RSD = 1.19% ） ,96. 5% （RSD = 1.32% ）. Conclusion： The method is convenient , accurate and reproducible , which can be used in quality control of kangshuai mixtura.