HPLC-ELSD法测定载银杏内酯B的PEG-PLGA纳米粒的包封率和载药量

目的建立PEG-PLGA纳米粒中银杏内酯B（GB）的包封率和载药量测定的HPLC-ELSD方法。方法色谱条件为：Shiseidoc8柱（250mm×4．6mm，5μm）；V（甲醇）：V（四氢呋喃）：V（水）=31：11：58为流动相；蒸发光散射检测器检测参数：蒸发口温度105℃，载气（N2）流速1．2L·min^-1，柱温45℃。应用低温超速离心法和凝胶过滤相结合的方法纯化纳米粒。结果本次建立的HPIE-EISD方法在GB质量浓度50—500mg·L^-1内线性关系良好，r=0．9991；重复性试验RSD为4．28％；平均回收率为95％-105％；载GB的PFG-PLGA纳米粒中杂质对GB的测定无干扰。GB的包封率为75．33％，载药量为3．77％。结论该方法简单、快速、准确，可用于载GB的PEG-PLGA纳米粒中GB包封率和载药量的测定。

HPLC-ELSD method to determine encapsulating efficiency and loading capacity of ginkgolide B in PEG-PLGA nanoparticles

A/M To establish a HPLC method with evaporative light scattering detector （ELSD） to determine the encapsulating efficiency and loading capacity of ginkgolide B（GB） in poly（ethyleneglycol） -poly（ L-lactic-co-glycolic acid） （PEG-PLGA） nanoparticles. METHODS Chromatographic conditions were as follows： Shiseido C8 column （250 mm ×4.6 mm, 5 μn） ; V（ methanol） ： V（ tetrahydrofuran ）： V（water ） = 31 ： 11：58 as mobile phase. ELSD parameters were set as follows： evaporation temperature was 105℃, carrier gas （N2） flow rate was 1.2 L·min-1 and column temperature was 45℃. Low temperature ultracentrifugation and gel filtration methods were used to purify nanoparticles. RESULTS In the range of 50 - 500 mg·L^-1, all plots were linear and the correlation coefficients were high than 0.999 0; the relative standard deviation （RSD） of the reproducibility was 4.28 % ; average recovery was in the range of 95 % - 105 %. As the dosage of GB was 1 mg, the encapsulating efficiency（EE） and the loading capacity（LC） was 75.33% and 3.77%, respectively. CONCLUSION The method is simple, rapid, accurate and can be used for quality control of GB loaded nanopartieles.