放射诱导基因Gadd 45在健康人群中表达水平的 检测及其意义

目的 考察Gadd 45基因在健康人群个体间表达的差异,评估Gadd 45发展成为一种新的辐射生物剂量计的可行性。方法 采集20例健康献血员血液样本,提取白细胞RNA进行反转录,以β-actin作为内对照基因,建立实时定量聚合酶链式反应(PCR)方法,并以此方法探讨人群中Gadd 45基因表达水平的差异。结果 20例受试者血液标本中的Gadd 45表达水平之间存在较大差异,且其表达水平与性别无关。结论 放射诱导基因Gadd 45的表达可能不适合做生物剂量计。

Expression of irradiation inducible gene Gadd 45 in population

Objective To investigate the difference of Gadd 45 expression in healthy population, and to evaluate the possibility of developing Gadd 45 expression into a new radiation bio-dosimeter. Methods Twenty blood samples from healthy population were collected and the total RNA was extracted from white blood cells and reverse transcribed into cDNA. The method of real-time PCR was used to detect the different level of Gadd 45 expression in human population, with β-actin as an internal control. Results There was a significant difference in Gadd 45 expression among 20 subjects. In addition, the difference was irrelevant to the sex. Conclusion The expression of irradiation-inducible gene Gadd 45 might not be a good bio-dosimeter.