喘息性肺炎患儿外周血中共刺激分子CD28及淋巴细胞亚群的变化

目的：探讨喘息性肺炎患儿外周血中共刺激分子CD28和淋巴细胞亚群的表达及意义。方法：应用流式细胞仪（FCM）检测了喘息性肺炎患儿及正常儿童外周血中共刺激分子CD28和淋巴细胞亚群的表达。结果：喘息性肺炎组共刺激分子CD28表达水平与正常对照组差异无统计学意义（P〉0.05）。T细胞亚群分析显示,喘息性肺炎患儿CD8＋CD28＋较对照组升高（P〈0.05）,CD8＋CD28-、CD4＋CD28＋较对照组降低（P〈0.05）,CD4＋CD28-与对照组相比差异无统计学意义。喘息性肺炎组CD3＋、CD3＋CD4＋、CD4＋/CD8＋较正常组降低,差异有统计学意义（P〈0.01）,CD3-CD19＋表达明显升高,差异有统计学意义（P〈0.01）,CD3＋CD8＋差异无统计学意义（P〉0.05）。结论：共刺激分子CD28参与了喘息性肺炎的发病机制,阻断第二信号有望成为治疗喘息性肺炎的一条新途径。

The Change of Costimulatory Molecules CD28 Lymphocyte and its Subsets in Peripheral Blood of Patients with Asthmatic Pneumonia

Objective：To investigate the expression and significances of costimulatory molecules CD28、lymphocyte and its subsets in peripheral blood of patients with asthmatic pneumonia.Method：Applying flow cytometry（FCM） to quantitatively analyze CD28、lymphocyte and its subsets.Results：There was no significant different between patients with asthmatic pneumonia and the normal controls on the expression of costimulatory molecules CD28（P0.05）. T lymphocyte and its subsets showed that CD8＋CD28＋ in patients with asthmatic pneumonia was more higher than the normal controls（P0.01）,CD8＋CD28-、 CD4＋CD28＋ were more lower（P0.05）.And there was no significant different on CD4＋CD28-between them.CD3＋、CD3＋CD4＋、CD4＋/CD8＋ in patients with asthmatic pneumonia was more lower than the normal controls（P0.01）,CD3-CD19＋ were more higher（P0.01）,CD3＋CD8＋ had no significant different between them.Conclusion：The costimulatory molecule CD28 play a critical role in the pathogenesis of asthmatic pneumonia.Blocking the costimulatory pathway appears to be a promising approach of the prevention and therapy of asthmatic pneumonia.