Surface plasma modification and tropoelastin coating of a polyurethane co-polymer for enhanced cell attachment and reduced thrombogenicity |
| |
Authors: | Daniel V. Bax Alexey Kondyurin Anna Waterhouse David R. McKenzie Anthony S. Weiss Marcela M.M. Bilek |
| |
Affiliation: | 1. Applied and Plasma Physics, School of Physics, University of Sydney, NSW 2006, Australia;2. School of Molecular Bioscience, University of Sydney, NSW 2006, Australia;3. Wyss Institute for Biologically Inspired Engineering, Harvard, Boston, MA 02115, USA;4. Bosch Institute, University of Sydney, NSW 2006, Australia;5. Charles Perkins Centre, University of Sydney, NSW 2006, Australia |
| |
Abstract: | Polymers currently utilized for dermal and vascular applications possess sub-optimal biocompatibility which reduces their efficacy. Improving the cell-binding and blood-contacting properties of these polymers would substantially improve their clinical utility. Tropoelastin is a highly extensible extracellular matrix protein with beneficial cell interactive and low thrombogenic properties. We transferred these benefits to the polyurethane block copolymer Elast-Eon E2A through a specific combination of surface plasma modifications and coating with human tropoelastin. The cell-binding activity of bound tropoelastin was modulated by ion implantation of the underlying polymer, and correlated with surface hydrophobicity, carbon and oxygen content. This combined treatment enhanced human dermal fibroblast (HDF) and human umbilical vein endothelial cell (HUVEC) attachment, cytoskeletal assembly and viability, combined with elevated PECAM-1 staining of HUVEC cell junctions. The thrombogenicity of the polymer was ameliorated by tropoelastin coating. We propose that a combination of metered plasma treatment and tropoelastin coating of Elast-Eon can serve to improve the biological performance of implantable devices such as vascular conduits. |
| |
Keywords: | Ion implantation Elast-Eon Block copolymer PDMS Polyurethane Plasma immersion ion implantation |
本文献已被 ScienceDirect 等数据库收录! |
|