1,25(OH)2D3 suppresses COX-2 up-regulation and thromboxane production in placental trophoblast cells in response to hypoxic stimulation |
| |
| Authors: | J. Sun W. Zhong Y. Gu L.J. Groome Y. Wang |
| |
| Affiliation: | 1. Dept. of Obstetrics and Gynecology, Louisiana State University Health Sciences Center, PO Box 33932, Shreveport, LA 71130, USA;2. Dept. of Obstetrics and Gynecology, First Hospital, Harbin Medical University, Harbin, China |
| |
| Abstract: | In this study, we determined if vitamin D could inhibit oxidative stress-induced thromboxane production by placental trophoblasts. Trophoblast isolated from normal placentas were stimulated with CoCl2, a hypoxic mimicking agent, with or without pretreatment of 1,25(OH)2D3. Soluble phospholipase-A2, metabolites of thromboxane-A2 and prostacyclin, and 8-isoprostane were measured. Expression of cyclooxygenase-1 (COX-1), COX-2, and heme oxygenase-1 (HO-1) were determined. We found that pretreatment of trophoblasts with 1,25(OH)2D3 significantly reduced 8-isoprostane and the ratio of thromboxane-A2 to prostacyclin production, and blocked COX-2 expression induced by CoCl2. These results provide evidence of the beneficial effects of vitamin D on placental trophoblasts. |
| |
| Keywords: | Vitamin D COX-2 Thromboxane Trophoblast |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
