毛细管区带电泳电化学检测人体尿样中的双氯灭痛、氯丙嗪

目的建立毛细管区带电泳法测定人体尿样中双氯灭痛、氯丙嗪含量的方法。方法采用弹性石英毛细管（31.5cm,25μmi.d.,360μmo.d.）,以4.90×10-3mol/LNa2HPO4-7.80×10-3mol/LNaH2PO4（pH=7）为缓冲液,10kV分离电压,5kV电渗进样10s,碳纤维电极工作电极（长200μm,直径8μm）,检测电势0.72V。结果双氯灭痛、氯丙嗪两种药物分别在9.90×10-6～5.00×10-4mol/L（r=0.9982）、5.0×10-7～1.0×10-4mol/L（r=0.9998）范围内表现出良好的线性,其回收率分别为104%,96.0%。结论该方法简便、快速、准确,可作为人体尿样中双氯灭痛、氯丙嗪的分离检测方法。

Determination of diclofenac sodium and chlorpromazine in human urine by capillary zone electrophoresis with electrochemical detection

Objective To establish the methods of determination of diclofenac sodium and chlorpromazine in human urine by capillary zone electrophoresis.Methods Quartz capillary column(31.5 cm,25μm i.d.,360 μm o.d.) was adopted.The optimum conditions of separation and detection were 4.90×10-3 mol/L Na2HPO4-7.80×10-3 mol/L NaH2PO4(pH = 7) for the buffer solution,10 kV for the separation voltage,5 kV and 10 s for the injection voltage and the injection time,0.72 V for the detection potential,respectively.Carbon fiber microelectrode(length:200 μm,diameter:8 μm).Results Diclofenac sodium and chlorpromazine had better linery relation in the range of 9.90×10-6-5.00×10-4 mol/L(r = 0.998 2),5.0×10-71.0×10-4 mol/L(r = 0.999 8),and the recovery was 104%,96.0%,respectively.Conclusion The method is simple,rapid,accuracy and is applied to the determination of diclofenac sodium and chlorpromazine in human urine.