| 阿胶、藕节、天花粉等氨基酸薄层层析方法研究与改进 |
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| 引用本文： | 张秋,李云霞,胡晓梅,段树卿,商春丽,于倩. 阿胶、藕节、天花粉等氨基酸薄层层析方法研究与改进[J]. 中国当代医药, 2012, 0(29): 66-67,69 |
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| 作者姓名： | 张秋 李云霞 胡晓梅 段树卿 商春丽 于倩 |
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| 作者单位： | 承德颈复康药业集团有限公司 |
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| 摘 要： | 目的改善和提高氨基酸薄层层析的分离效果。方法采用盐酸﹕水（1：1）提取,以正丙醇-36%乙酸（7：3）为展开剂,含1%茚三酮的丙酮溶液为显色剂,105℃加热显色至斑点显色清晰。结果色谱斑点清晰,分离理想,各斑点Rf值适中。结论本方法较《中华人民共和国药典》中关于氨基酸的薄层层析的方法简单,重复性好,具有较好的可行性和实用性。
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| 关 键 词： | 阿胶 鹿角胶 地龙 天花粉 半夏 藕节 薄层层析 |
Study and improvement of thin layer chromatography method on Amino acid of Donkey-hide glue,Rhizoma nelumbinis,Radix trichosanthis,etc |
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| ZHANG Qiu,LI Yunxia,HU Xiaomei,DUAN Shuqing,SHANG Chunli,YU Qian. Study and improvement of thin layer chromatography method on Amino acid of Donkey-hide glue,Rhizoma nelumbinis,Radix trichosanthis,etc[J]. http://www.botanicus.org/, 2012, 0(29): 66-67,69 |
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| Authors: | ZHANG Qiu LI Yunxia HU Xiaomei DUAN Shuqing SHANG Chunli YU Qian |
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| Affiliation: | Chengde Jingfukang Pharmaceutical Group Co.,LTD in Hebei Province,Chengde 067000,China |
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| Abstract: | Objective To improve and enhance the separation effect of thin layer chromatography method on amino acid.Methods Extracted with hydrochloric acid﹕water(1:1),n-propyl alcohol-36% acetic acid(7:3) as developing solvent,1% ninhydrin as spray reagent,heated the spot to 105℃ until it could be observed clearly.Results The tincture flecks appeared clearly on the chromatographic chart and the Rf value was perfect.The separation effect was good.Conclusion This method is easier than the one in "Chinese Pharmacopoeia".It is feasible,practical,and repeatable. |
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| Keywords: | Donkey-hide glue Antler gum Earthworm Radix trichosanthis Pinellia ternata Rhizoma nelumbinis Thin layer chromatography method |
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