Effect of local cooling on pro-inflammatory cytokines and blood flow of the skin under surface pressure in rats: Feasibility study |
| |
| Affiliation: | 1. Department of Physiology, University of Oklahoma Health Sciences Center, Oklahoma City, OK, USA;2. Rehabilitation Engineering Laboratory, Department of Kinesiology and Community Health, University of Illinois at Urbana-Champaign, Champaign, IL, USA;1. Division of Pediatric Cardiology, Department of Pediatrics, Medical University of South Carolina, Charleston, SC;2. Department of Public Health Sciences, Medical University of South Carolina, Charleston, SC;3. Division of Neonatology, Department of Pediatrics, Medical University of South Carolina, Charleston, SC;1. Section of Anaesthesiology and Intensive Care, Department of Clinical Science and Education, Karolinska Institutet, Stockholm, Sweden;2. Institute of Physiology and Pharmacology, Medical Academy, Lithuanian University of Health Sciences, Kaunas, Lithuania;3. Department of Electronics Engineering, Kaunas University of Technology, Kaunas, Lithuania;4. Clinic of Anaesthesiology and Intensive Care, Faculty of Medicine, Vilnius University, Vilnius, Lithuania;1. Department of Rehabilitation Sciences, The Hong Kong Polytechnic University, Hong Kong Special Administrative Region;2. Physiotherapy Department, Kowloon Hospital, Hong Kong Special Administrative Region, China;3. Faculty of Arts and Sciences, The Hong Kong Institute of Education, Hong Kong Special Administrative Region, China;1. Department of Ophthalmology, University of Ulsan College of Medicine, Asan Medical Center, Seoul, Republic of Korea;2. Department of Ophthalmology, Kangbuk Samsung Hospital, Sungkyunkwan University School of Medicine, Seoul, Republic of Korea;3. Research Institute for Biomacromolecules, University of Ulsan College of Medicine, Asan Medical Center, Seoul, Republic of Korea;1. Department of Biochemistry, University of Utah, Salt Lake City, Utah 84112-5650;2. Arcturus Therapeutics, San Diego, California 92121;3. Cardiovascular Center, Medical College of Wisconsin, Milwaukee, Wisconsin 53226;5. Departments of Anesthesiology and Pharmacology and Toxicology, Medical College of Wisconsin, Milwaukee, Wisconsin 53226;4. Department of Chemistry, University of Oxford, Oxford OX1 3TA, United Kingdom;6. Department of Pathophysiology, Xiangya School of Medicine, Central South University, Changsha City, Hunan 410078, China;7. Division of Cardiology, Department of Medicine, University of Utah, Salt Lake City, Utah 84132;8. Northern Genetics Service, Newcastle upon Tyne Hospitals NHS Foundation Trust, Newcastle upon Tyne NE1 3BZ, United Kingdom;9. National Health Service England Health Science Services for Rare Neuromuscular Diseases, Muscle Immunoanalysis Unit, Dental Hospital, Richardson Road, Newcastle upon Tyne NE2 4AZ, United Kingdom;10. Neuromuscular Genetics, Newcastle University John Walton Centre for Muscular Dystrophy Research, Medical Research Council Centre for Neuromuscular Diseases, Institute of Genetic Medicine, International Centre for Life, Newcastle upon Tyne NE1 3BZ, United Kingdom;11. Department of Paediatric Neurology, Neuromuscular Service Evelina Children''s Hospital, Guy''s and St. Thomas'' National Health Service Foundation Trust, London SE1 7EH, United Kingdom;12. Randall Division of Cell and Molecular Biophysics, Muscle Signalling Section, King''s College London, London SE1 1UL, United Kingdom;13. Department of Basic and Clinical Neuroscience Institute of Psychiatry, Psychology and Neuroscience, King''s College London SE5 9RX, United Kingdom;14. PharmaCell, Maastricht, Netherlands, 6229 EV Maastricht;15. Sorbonne Universités, University Pierre and Marie Curie, Univ Paris 06, CNRS, Laboratoire de Biologie du Dévelopment de Villefranche sur mer (LBDV), UMR 7009, 181 Chemin du Lazaret, 06230 Villefranche sur mer, France;p. Blood Research Institute, BloodCenter of Wisconsin, Milwaukee, Wisconsin 53226 |
| |
| Abstract: | The primary purpose of this feasibility study was to establish a correlation between pro-inflammatory cytokine accumulation and severity of tissue damage during local pressure with various temperatures. The secondary purpose was to compare skin blood flow patterns for assessing the efficacy of local cooling on reducing skin ischemia under surface pressure. Eight Sprague–Dawley rats were assigned to two protocols, including pressure with local cooling (Δt = −10 °C) and pressure with local heating (Δt = 10 °C). Pressure of 700 mmHg was applied to the right trochanter area of rats for 3 h. Skin perfusion quantified by laser Doppler flowmetry and TNF-1 and IL-1β levels were measured. Our results showed that TNF-α concentrations were increased more significantly with local heating than with local cooling under pressure whereas IL-1β did not change. Our results support the notion that weight bearing soft tissue damage may be reduced through temperature modulation and that non-invasive perfusion measurements using laser Doppler flowmetry may be capable of assessing viability. Furthermore, these results show that perfusion response to loading pressure may be correlated with changes in local pro-inflammatory cytokines. These relationships may be relevant for the development of cooling technologies for reducing risk of pressure ulcers. |
| |
| Keywords: | Cooling Cytokines Inflammation Laser Doppler Pressure ulcers |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
