职业紧张和AGT基因对高血压患病率的影响

目的 探讨职业紧张和AGT M235T和T174M位点多态性对新疆油田工人高血压患病率的影响。方法 采用随机整群抽样方法抽取克拉玛依5个油田作业分公司的1 200名工人并分发问卷，回收有效问卷1 156份（有效率为96.33%）。运用聚合酶链式反应限制性片段长度多态性（PCR - RFLP）技术检测高血压病例组和对照组（各153例）AGT M235T和T174M位点的基因型。结果 油田工人中男性、年龄＜45岁，工龄≤15年、轮班作业、大专及以上文化程度、未婚、吸烟和饮酒组的职业紧张程度较高（P＜0.05），且高血压患病率随着职业紧张程度的增加而升高（χ2 = 16.959，P＜0.001）；病例组的AGT M235T位点TT基因型较多（P＜0.05）；T174M位点的基因型在病例组和对照组的差异无统计学意义（P＞0.05）。二元logistic回归结果显示M235T位点的的MM、MT基因型和个体紧张反应是高血压的保护因素，TT基因型和高度职业紧张程度是其危险因素。 结论 AGT M235T位点的TT基因型、较高职业紧张和较低的个体紧张反应会增加高血压的发病风险；AGT T174M位点与本研究人群的高血压无关。

Effects of occupational stress and AGT gene on the prevalence of hypertension

Objective To investigate the effects of occupational stress and AGT M235T and T174M polymorphisms on the prevalence of hypertension in Xinjiang oilfield workers. Methods A random cluster sampling method was used to select 1 200 workers from 5 oilfield operating branches in Karamay, and their occupational stress were investigated by occupational stress measurement questionnaire. In total 1 156 valid questionnaires were collected, and the effective rate of the questionnaire was 96.33%. The genotypes of AGT gene M235T and T174M in the hypertensive case group and control group (153 cases in each) were detected by polymerase chain reaction-restriction fragment length polymorphism. Results Among the oilfield workers, the occupational stress degree of male, age <45 years old, working age ≤15 years, shift workers, college education level or above, unmarried, smoking and drinking group was higher than that of other corresponding groups (P<0.05). The prevalence of hypertension in oilfield workers increased with the increase of occupational stress (χ2=16.959, P<0.001). There were statistically significant differences in MM, MT and TT genotypes of AGT M235T between the case group and the control group (P<0.05), and there were more TT genotypes in the case group. There was no significant difference in the genotype of the T174M between the case group and the control group (P>0.05). The results of binary Logistic regression showed that the MM and MT genotypes of M235T and individual stress response were protective factors for hypertension, while the TT genotype and high occupational stress were risk factors for hypertension. Conclusion The TT genotype of AGT M235T, higher occupational stress and lower individual stress response increase the risk of hypertension. AGT T174M is not associated with hypertension.