Impact of Implementation of an Automated Liquid Culture System on Diagnosis of Tuberculous Pleurisy |
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| Authors: | Byung Hee Lee Seong Hoon Yoon Hye Ju Yeo Dong Wan Kim Seung Eun Lee Woo Hyun Cho Su Jin Lee Yun Seong Kim Doosoo Jeon |
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| Affiliation: | 1.Department of Internal Medicine, Pusan National University School of Medicine, Yangsan, Korea.;2.Research Institute for Convergence of Biomedical Science and Technology, Pusan National University Yangsan Hospital, Yangsan, Korea. |
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| Abstract: | This study was conducted to evaluate the impact of implementation of an automated liquid culture system on the diagnosis of tuberculous pleurisy in an HIV-uninfected patient population. We retrospectively compared the culture yield, time to positivity, and contamination rate of pleural effusion samples in the BACTEC Mycobacteria Growth Indicator Tube 960 (MGIT) and Ogawa media among patients with tuberculous pleurisy. Out of 104 effusion samples, 43 (41.3%) were culture positive on either the MGIT or the Ogawa media. The culture yield of MGIT was higher (40.4%, 42/104) than that of Ogawa media (18.3%, 19/104) (P<0.001). One of the samples was positive only on the Ogawa medium. The median time to positivity was faster in the MGIT (18 days, range 8-32 days) than in the Ogawa media (37 days, range 20-59 days) (P<0.001). No contamination or growth of nontuberculous mycobacterium was observed on either of the culture media. In conclusion, the automated liquid culture system could provide approximately twice as high yields and fast results in effusion culture, compared to solid media. Supplemental solid media may have a limited impact on maximizing sensitivity in effusion culture; however, further studies are required.Graphical Abstract | |
| Keywords: | Tuberculosis Pleural Effusion Pleurisy Culture Media |
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