噪声对小鼠血糖、炎症因子及肠道菌群的影响

目的 探讨噪声对小鼠血糖、炎症因子及肠道菌群的影响。方法 32只雄性 C57BL/6J小鼠，每组8只，随机分为15 d对照组、15 d噪声组、30 d对照组和30 d噪声组。干预结束后进行腹腔注射葡萄糖耐量试验，用ELISA法检测血清TNF - α、TNF - γ、IL - 6、IL - 1β、IFN - γ水平，16S rRNA高通量测序技术检测粪便菌群。结果 与对照组相比，噪声组的体重变化无明显差异（P＞0.05）；葡萄糖耐量试验曲线下面积（AUC）显著升高（P＜0.05），糖耐量减退；血清TNF - α、TNF - γ、IL - 6、IL - 1β、IFN - γ水平也明显升高（P＜0.05）。肠道菌群结果显示，与各对照组相比，各噪声组小鼠肠道菌群的α多样性均降低（P＜0.05），β多样性的主成分分析（PCoA）图明显的区分。所有组别均以拟杆菌门（Bacteroidetes）和厚壁菌门（Firmicutes）为主，噪声组的Bacteroidetes、普雷沃菌属（Prevotella）增加，Firmicutes、疣微菌门（Verrucomicrobia）、阿克曼菌属（Akkermansia）和瘤胃球菌属（Ruminococcus）减少。α多样性指数chao1、faith_pd、shannon与AUC均呈负相关，Bacteroidetes与TNF - α呈正相关，Firmicutes与AUC、TNF - α、IL - 6呈负相关，Prevotella与AUC、TNF - α呈正相关。结论 噪声暴露能引起小鼠炎症反应增强和肠道菌群改变，导致糖耐量减退。

Effects of noise on blood glucose,inflammatory factors and intestinal flora of mice

Objective To explore the effects of noise exposure on blood glucose, inflammatory factors and intestinal flora of mice. Methods Thirty-two male C57BL/6J mice were randomly divided into 15-day control group (con_15d), 15-day noise group (noise_15d), 30-day control group (con_30d) and 30-day noise group (noise_30d), with 8 in each group. After 30 days of intervention, IGTT test was performed, serum TNF-α, TNF-γ, IL-6, IL-1β and IFN-γ levels were detected by ELISA, and fecal flora was detected by 16s rRNA high-throughput sequencing technology. Results Compared with the control groups, there was no significant difference in body weight changes in the noise groups (P>0.05); the area under the IGTT test curve (AUC) was significantly increased (P<0.05), and the glucose tolerance was impaired; serum TNF-α, TNF-γ, IL-6, IL-1β and IFN-γ levels were also significantly increased (P<0.05). The results of intestinal flora analysis showed that compared with control groups, the α diversity of the intestinal flora in noise groups were reduced (P<0.05), and the principal component analysis (PCoA) diagrams of the β diversity between noise groups and control groups also presented clear distinction. The composition of the intestinal flora in all groups was dominated by Bacteroidetes and Firmicutes, and Bacteroidetes and Prevotella in the noise groups were increased, but Firmicutes, Verrucomicrobia, Akkermansia and Ruminococcus were decreased. The α diversity (chao1, faith_pd and shannon index) were found to be inversely correlated with AUC, Bacteroidetes was positively correlated with TNF-α, and Firmicutes was inversely correlated with AUC, TNF-α and IL-6, and Prevotella was also inversely correlated with AUC and TNF-α. Conclusion Noise exposure causes increased inflammatory response and alters intestinal flora in mice, resulting in decreased glucose tolerance.